Nutrition Department of Para-Medical School, Ahvaz Jondishapur University of Medical Sciences, Ahvaz 61357-15794, Iran.
J Clin Biochem Nutr. 2009 Nov;45(3):285-91. doi: 10.3164/jcbn.09-15. Epub 2009 Oct 28.
Our objective was to examine the effect of orange juice and hesperetin on serum total antioxidant capacity (TAC), lipid peroxidation (MDA), uric acid and hepatic xanthine oxidase (XO) and xanthine dehydrogenase (XDH) activity in hyperuricemic rats. Experimentally hyperuricemia in rats was induced by intraperitoneal injection of potassium oxonate (250 mg/kg). Orange juice (5 ml/kg) and hesperetin (5 mg/kg) was given by oral gavage to rats for 2 weeks and biochemical data was measured. Data showed that orange juice supplementation increased serum TAC and decreased MDA concentration (p</=0.05). Orange juice also inhibited hepatic XO and XDH activity and decreased serum uric acid levels. Hesperetin, which is the main flavanone constituent in orange juice, also exhibited antioxidative and antihyperuricemic properties, but its effect was weaker than that of orange juice. Although the hypouricemic effect of allopurinol (5 mg/kg), as a positive control, was much higher than that of orange juice and hesperetin, it could not significantly change biomarkers of oxidative stress. These features of orange juice and hesperetin make them an attractive candidate for the prophylactic treatment of hyperuricaemia, particularly if these compounds are to be taken on a long-term basis.
我们的目的是研究橙汁和橙皮苷对高尿酸血症大鼠血清总抗氧化能力（TAC）、脂质过氧化（MDA）、尿酸、肝黄嘌呤氧化酶（XO）和黄嘌呤脱氢酶（XDH）活性的影响。实验性高尿酸血症大鼠通过腹腔注射氧嗪酸钾（250mg/kg）诱导。橙汁（5ml/kg）和橙皮苷（5mg/kg）经口灌胃给予大鼠 2 周，并测定生化数据。结果表明，橙汁补充可增加血清 TAC，降低 MDA 浓度（p</=0.05）。橙汁还抑制肝 XO 和 XDH 活性，降低血清尿酸水平。橙皮苷是橙汁中主要的黄烷酮成分，也具有抗氧化和抗高尿酸血症作用，但作用弱于橙汁。虽然阳性对照药别嘌呤醇（5mg/kg）的降尿酸作用明显高于橙汁和橙皮苷，但不能显著改变氧化应激的生物标志物。橙汁和橙皮苷的这些特性使它们成为高尿酸血症预防治疗的有吸引力的候选药物，特别是如果这些化合物需要长期服用。
