Gayathri Mahalingam, Kannabiran Krishnan
Biomolecules and Genetics Division, School of Biotechnology, Chemical and Biomedical Engineering, VIT University, Vellore, Tamil Nadu, India.
Int J Diabetes Dev Ctries. 2008 Jan;28(1):6-10. doi: 10.4103/0973-3930.41979.
To evaluate the antidiabetic activity of an aqueous extract of the roots of Hemidesmus indicus on blood glucose, serum electrolytes, serum marker enzymes, liver microsomal P-450 enzymes, and lipid peroxidation in the liver and kidney of streptozotocin-induced diabetic rats.
Effect of H. indicus extract on blood glucose was studied with fed, fasted and glucose-loaded diabetic and nondiabetic rat models. The effect of the extract on serum electrolytes, serum levels of key glucose metabolizing enzymes, hepatic microsomal protein and hepatic cytochrome P-450-dependent mono-oxygenase enzyme systems and lipid peroxidation in the liver and kidney of diabetic rats. One way analysis of variance and Duncan's multiple range test was used for statistical analysis.
Oral administration of H. indicus aqueous extract to fed, fasted and glucose-loaded diabetic rats decreased blood glucose level significantly at 5 h and restored serum electrolytes, glycolytic enzymes and hepatic cytochrome P-450-dependent enzyme systems by preventing the formation of liver and kidney lipid peroxides at the end of 12 weeks of the study period.
From the studies, it can be concluded that the aqueous extract of the roots of H. indicus at a dosage of 500 mg/kg/day exhibits significant antidiabetic activity. It restores the concentrations of electrolytes, glucose metabolizing enzymes, hepatic microsomal protein and hepatic cytochrome P-450-dependent mono-oxygenase enzyme systems to near normal level and also corrects the related metabolic alterations in experimentally induced diabetic rats. H. indicus administration also decreased liver and kidney lipid peroxidation products. On the basis of our findings, H. indicus could be used as an antidiabetic and antioxidant agent for the prevention and treatment of diabetes mellitus.
评估印度牛弥菜根水提取物对链脲佐菌素诱导的糖尿病大鼠的血糖、血清电解质、血清标志物酶、肝微粒体P - 450酶以及肝和肾脂质过氧化的抗糖尿病活性。
采用喂食、禁食和葡萄糖负荷的糖尿病及非糖尿病大鼠模型，研究印度牛弥菜提取物对血糖的影响。研究该提取物对糖尿病大鼠血清电解质、关键葡萄糖代谢酶的血清水平、肝微粒体蛋白、肝细胞色素P - 450依赖性单加氧酶系统以及肝和肾脂质过氧化的影响。采用单因素方差分析和邓肯多重极差检验进行统计分析。
在研究期12周结束时，给喂食、禁食和葡萄糖负荷的糖尿病大鼠口服印度牛弥菜水提取物，可在5小时时显著降低血糖水平，并通过防止肝和肾脂质过氧化物的形成来恢复血清电解质、糖酵解酶和肝细胞色素P - 450依赖性酶系统。
从这些研究中可以得出结论，印度牛弥菜根水提取物以500mg/kg/天的剂量具有显著的抗糖尿病活性。它能将电解质、葡萄糖代谢酶、肝微粒体蛋白和肝细胞色素P - 450依赖性单加氧酶系统的浓度恢复到接近正常水平，并纠正实验性诱导糖尿病大鼠的相关代谢改变。给予印度牛弥菜还可降低肝和肾脂质过氧化产物。基于我们的研究结果，印度牛弥菜可作为预防和治疗糖尿病的抗糖尿病和抗氧化剂。
