氧化石墨烯放大量子点的电致化学发光及其对含巯基化合物中谷胱甘肽的选择性传感。
Graphene oxide amplified electrogenerated chemiluminescence of quantum dots and its selective sensing for glutathione from thiol-containing compounds.
机构信息
Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Tsinghua University, Beijing 100084, China.
出版信息
Anal Chem. 2009 Dec 1;81(23):9710-5. doi: 10.1021/ac901935a.
Here we report a graphene oxide amplified electrogenerated chemiluminescence (ECL) of quantum dots (QDs) platform and its efficient selective sensing for antioxidants. Graphene oxide facilitated the CdTe QDs*+ production and triggered O2*- generation. Then, a high yield of CdTe QDs* was formed due to the combination of CdTe QDs*+ and O2*-, leading to an approximately 5-fold ECL amplification. Glutathione is the most abundant cellular thiol-containing peptide, but its selective sensing is an intractable issue in analytical and biochemical communities because its detection is interfered with by some thiol-containing compounds. This platform showed a detection limit of 8.3 microM (S/N = 3) for glutathione and a selective detection linear dependence from 24 to 214 microM in the presence of 120 muM cysteine and glutathione disulfide. This platform was also successfully used for real sample (eye drug containing glutathione) detection without any pretreatment with a wide linear range from 0.04 to 0.29 microg mL(-1).
在这里，我们报告了一种基于氧化石墨烯放大的量子点（QD）电化学发光（ECL）平台，及其对抗氧化剂的高效选择性传感。氧化石墨烯促进了 CdTe QD*+的产生，并引发了 O2*-的生成。然后，由于 CdTe QD*+和 O2*-的结合，形成了大量的 CdTe QDs*，导致 ECL 信号大约放大了 5 倍。谷胱甘肽是细胞中最丰富的含巯基肽，但由于其检测受到一些含巯基化合物的干扰，因此在分析和生化领域中对其进行选择性检测是一个难题。该平台对谷胱甘肽的检测限为 8.3 μM（S/N = 3），在存在 120 μM 半胱氨酸和谷胱甘肽二硫化物的情况下，从 24 到 214 μM 呈现出选择性检测的线性依赖性。该平台还成功地用于真实样品（含有谷胱甘肽的眼药）检测，无需任何预处理，线性范围从 0.04 到 0.29 μg mL(-1)。
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