Institute for Stem cell Therapy and Exploration of Monogenic diseases, Desbruères, 91030 Evry cedex, France.
Regen Med. 2009 Nov;4(6):899-909. doi: 10.2217/rme.09.63.
Owing to their original properties, pluripotent human embryonic stem cells (hESCs) and their progenies are highly valuable not only for regenerative medicine, but also as tools to study development and pathologies or as cellular substrates to screen and test new drugs. However, ensuring their genomic integrity is one important prerequisite for both research and therapeutic applications. Until recently, several studies about the genomic stability of cultured hESCs had described chromosomal or else large genomic alterations detectable with conventional karyotypic methods. In the past year, several laboratories have reported many small genomic alterations, in the megabase-sized range, using more sensitive karyotyping methods, showing that hESCs are prone to acquire focal genomic abnormalities in culture. As these alterations were found to be nonrandom, these findings strongly advocate for high-resolution monitoring of human pluripotent stem cell lines, especially when intended to be used for clinical applications.
由于其原始特性，多能人类胚胎干细胞（hESCs）及其后代不仅对于再生医学非常有价值，而且还可用作研究发育和病理学的工具，或者用作筛选和测试新药的细胞基质。然而，确保其基因组完整性是研究和治疗应用的一个重要前提。直到最近，几项关于培养的 hESC 基因组稳定性的研究已经描述了可通过常规核型方法检测到的染色体或其他大型基因组改变。在过去的一年中，几个实验室使用更敏感的核型分析方法报告了许多大小在兆碱基范围内的小型基因组改变，表明 hESC 在培养中容易获得局灶性基因组异常。由于这些改变是非随机的，这些发现强烈主张对人类多能干细胞系进行高分辨率监测，特别是当打算将其用于临床应用时。
