Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Wound Repair Regen. 2009 Nov-Dec;17(6):806-16. doi: 10.1111/j.1524-475X.2009.00546.x.
ABSTRACT Decreased inflammatory response seen in wet wound healing may be correlated with diminished scarring. This study seeks to test this hypothesis and to validate a model of scarring in the Yorkshire pig. Four Yorkshire pigs were used to create 36 dorsal wounds per pig (144 wounds total) in the following groups: full-thickness excisional, partial thickness, meshed split-thickness skin grafts, sheet split-thickness skin grafts, minced skin, and incisional wounds. Wounds were randomized into wet and dry groups. Wet wounds were enclosed in polyurethane chambers with 2 mL of normal saline. Dry wounds were covered with regular gauze. Terminal biopsies were performed at 72 hours and day 28. Histology demonstrated significantly less inflammatory infiltrate, thicker neoepidermis, more pronounced rete ridge formation, and decreased scar tissue thickness in wet wounds. The mean macroscopic scar surface area was significantly decreased in full-thickness excisional wet wounds compared with dry wounds (61.2 mm(2) vs. 150.8 mm(2), p<0.01). Hydroxyproline content was decreased in full-thickness wet compared with dry groups (44.81 vs. 62.21 mg/g, p<0.01). Tensile strength was 90% greater in full-thickness wet compared with dry groups (p<0.01). Healing in the liquid environment significantly reduced scar formation. This model will allow for future investigation of high-concentration topical scar-modulating agents in the liquid environment.
湿性伤口愈合中观察到的炎症反应减少可能与疤痕减少有关。本研究旨在验证这一假说，并验证在约克夏猪中疤痕形成的模型。使用四只约克夏猪在每只猪的背部创建 36 个全层切开伤（总共 144 个伤口），分为以下几组：全层切开伤、部分厚度、网眼状切取的皮肤移植物、片状切取的皮肤移植物、切碎的皮肤和切开伤。伤口随机分为湿性和干性组。湿性伤口用含有 2 毫升生理盐水的聚氨酯室封闭。干性伤口用普通纱布覆盖。在 72 小时和第 28 天进行终端活检。组织学显示，湿性伤口的炎症浸润明显减少，新生表皮较厚，网纹嵴形成更明显，疤痕组织厚度减少。与干性伤口相比，全层切开湿性伤口的平均宏观疤痕表面积显著减小（61.2mm2 比 150.8mm2，p<0.01）。全层湿性组的羟脯氨酸含量较干性组降低（44.81 比 62.21mg/g，p<0.01）。与干性组相比，全层湿性组的拉伸强度增加了 90%（p<0.01）。在液体环境中的愈合显著减少了疤痕形成。该模型将允许未来在液体环境中对高浓度局部疤痕调节剂进行研究。
