Department of Animal Science, Faculty of Agriculture, University of Yüzüncü Yil, Van, Turkey.
J Anim Physiol Anim Nutr (Berl). 2010 Aug 1;94(4):519-26. doi: 10.1111/j.1439-0396.2009.00938.x. Epub 2009 Nov 11.
One-hundred and fifty male chickens were used to evaluate the effects of different activities (0, 250, 500, 12 500 FTU/kg) of phytase on their performance and antioxidant concentration in the liver. The chicks were housed in 30 cages and were allocated to six replicates of five dietary treatments. All diets were formulated to be adequate in energy and protein (12.90 MJ/kg metabolizable energy, 214 g/kg crude protein), however, the negative control (NC) was lower in available P compared with the positive control (PC) (2.5 vs. 4.5 g/kg diet). The other three diets were the NC supplemented with phytase at 250, 500 and 12 500 FTU/kg (NC + 250, NC + 500 and NC + 12 500 FTU respectively). The concentration of antioxidants in the liver of the birds was determined using HPLC at 21 days of age. Low P diets (NC) reduced weight gain, however, supplementation with phytase improved weight gain to the extent that it was better than the PC at the 12 500 FTU treatment (p < 0.05). Feed conversion ratio was also improved by the high level of phytase supplement more than other treatments (p < 0.05). Feed consumption was not affected either by dietary phosphorus concentration or by different phytase supplementation. The antioxidant data showed that the unsupplemented diet with low phosphorus (NC) decreased the concentration of coenzyme Q(10) and retinol-linoleate in the liver compared with that of birds on the adequate phosphorus treatment (PC). Phytase supplementation, especially at the higher doses (500 and 12 500 FTU) increased the level of coenzyme Q(10) to the same level as the PC treatment. In addition, the highest dose (12 500 FTU) of phytase increased retinol concentration in the liver of chickens compared with those on the NC treatment. The highest inclusion level of phytase increased the alpha-tocopherol level in the liver compared with the lower levels of phytase (NC + 250 and NC + 500 FTU).
一百五十只雄性小鸡被用于评估不同活性（0、250、500、12500FTU/kg）植酸酶对其性能和肝脏抗氧化剂浓度的影响。小鸡被安置在 30 个笼子中，并被分配到六个日粮处理的重复中。所有日粮的能量和蛋白质含量均充足（代谢能 12.90MJ/kg，粗蛋白 214g/kg），然而，与阳性对照（PC）相比，阴性对照（NC）的有效磷较低（2.5g/kg 日粮对 4.5g/kg 日粮）。另外三种日粮是 NC 分别添加 250、500 和 12500FTU/kg 植酸酶的 NC 补充日粮（NC+250、NC+500 和 NC+12500FTU）。21 日龄时，使用 HPLC 法测定鸟类肝脏中的抗氧化剂浓度。低磷日粮（NC）降低了体重增加，但添加植酸酶提高了体重增加的程度，在 12500FTU 处理时优于 PC（p<0.05）。高水平植酸酶补充剂也比其他处理更好地改善了饲料转化率（p<0.05）。日粮磷浓度或不同植酸酶补充剂均不影响饲料消耗。抗氧化剂数据显示，与磷充足处理（PC）相比，低磷未补充日粮（NC）降低了肝脏中辅酶 Q（10）和视黄醇亚油酸的浓度。植酸酶补充剂，尤其是高剂量（500 和 12500FTU），将辅酶 Q（10）水平提高到与 PC 处理相同的水平。此外，植酸酶的最高剂量（12500FTU）增加了鸡肝脏中的视黄醇浓度，与 NC 处理相比。植酸酶的最高添加水平与较低水平的植酸酶（NC+250 和 NC+500FTU）相比，增加了肝脏中的α-生育酚水平。
