College of Chemistry and Environmental Science, Henan Normal University, Xinxiang, China.
J Inorg Biochem. 2010 Jan;104(1):87-91. doi: 10.1016/j.jinorgbio.2009.10.015. Epub 2009 Oct 23.
In this paper, antineoplastic activities of protein-conjugated silver sulfide nano-crystals with different shapes were described in detail. Transmission electron microscope analysis demonstrated that stable and well-disperse protein-conjugated silver sulfide nano-particles, nano-rods, and nano-wires could be prepared by aqueous chemistry method. The Fourier transform infrared spectrograph analysis indicated the strong coordination between silver sulfide surfaces and -OH and -NH groups in bovine serum albumin. The antineoplastic activities of protein-conjugated silver sulfide nano-crystals were examined by cell viability analysis, optical and electron microscopy methods. The results showed that nano-particles, nano-rods and nano-wires could inhibit the proliferations of human hepatocellular carcinoma Bel-7402 cells and C6 glioma cells, and the activities were size-dependent.
本文详细描述了不同形状的蛋白结合硫化银纳米晶体的抗肿瘤活性。透射电子显微镜分析表明，通过水相化学法可以制备稳定且分散良好的蛋白结合硫化银纳米粒子、纳米棒和纳米线。傅里叶变换红外光谱分析表明，硫化银表面与牛血清白蛋白中的-OH 和 -NH 基团之间存在强烈的配位作用。通过细胞活力分析、光学显微镜和电子显微镜方法研究了蛋白结合硫化银纳米晶体的抗肿瘤活性。结果表明，纳米粒子、纳米棒和纳米线可以抑制人肝癌 Bel-7402 细胞和 C6 神经胶质瘤细胞的增殖，且其活性具有尺寸依赖性。
