Maestrini Michela, Molento Marcelo Beltrão, Mancini Simone, Martini Mina, Angeletti Francesco Giovanni Salvo, Perrucci Stefania
Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy.
Department of Veterinary Medicine, University of Paraná, R: dos Funcionarios, 1540, Curitiba 80035-050, PR, Brazil.
Vet Sci. 2020 Dec 2;7(4):195. doi: 10.3390/vetsci7040195.
Intestinal strongyles are common helminths of donkeys, in which they may be responsible for disease and poor performance. This study aimed to identify intestinal strongyle genera in 55 naturally infected donkeys from three different farm typologies in Tuscany, central Italy, using morphological and metrical analysis of third stage larvae (L3) obtained from faecal cultures. Larvae were identified using two previous reported morphological identification keys. Moreover, eggs per gram (EPG) data were also evaluated to assess differences, if any, according to the farm typology, sex, and age of the examined donkeys. The results showed that small strongyles were prevalent in all donkey farms. In all examined farms, most (92-100%) of L3 were identified as cyathostomin species of the genera spp. and spp. Large strongyles of the genera spp. and spp., were identified at low percentage (8%), only in the single organic farm included in the study. A high agreement was observed between the two different morphometric keys used. No significant differences were found for EPG according to farm typology, and sex and age from the examined donkeys. This is the first report about genera identification of intestinal strongyles infecting donkeys in Tuscany, Italy.
肠道圆线虫是驴常见的蠕虫，它们可能导致疾病和生长性能不佳。本研究旨在通过对从粪便培养物中获得的第三期幼虫（L3）进行形态学和测量分析，鉴定意大利中部托斯卡纳地区三种不同农场类型的55头自然感染驴的肠道圆线虫属。使用先前报道的两种形态学鉴定方法来鉴定幼虫。此外，还评估了每克粪便虫卵数（EPG）数据，以根据所检查驴的农场类型、性别和年龄评估是否存在差异。结果表明，小型圆线虫在所有养驴场中都很普遍。在所有检查的农场中，大多数（92%-100%）的L3被鉴定为圆线属和杯环线虫属的杯状类圆线虫物种。仅在本研究纳入的单个有机农场中，低比例（8%）地鉴定出了大型圆线虫属的马圆线虫属和无齿圆线虫属。所使用的两种不同形态测量方法之间观察到高度一致性。根据农场类型、所检查驴的性别和年龄，EPG未发现显著差异。这是关于意大利托斯卡纳地区感染驴的肠道圆线虫属鉴定的首份报告。
