Xia Xue-Kui, Huang Hua-Rong, She Zhi-Gang, Shao Chang-Lun, Liu Fan, Cai Xiao-Ling, Vrijmoed L L P, Lin Yong-Cheng
School of Chemistry and Chemical Engineering, Sun Yat-sen (Zhongshan) University, Guangzhou 510275, P. R. China.
Magn Reson Chem. 2007 Nov;45(11):1006-9. doi: 10.1002/mrc.2078.
We report the unambiguous assignments of the (1)H and (13)C NMR spectra of two new natural products, namely, 1,4,5,6,7,9-hexahydroxy-2-methoxy-7-methyl-5beta,9beta,8abeta, 6alpha,10aalpha-hexahydroanthracen-10 (10aH)-one (1) and 1,4,6-trihydroxy-2-methoxy-7-methylanthracene-9, 10-dione (2), together with three known anthraquinones. These compounds were all isolated from the marine endophytic fungus No. 1403 collected from the South China Sea. Compounds 3 and 4 were isolated from the marine fungus for the first time. The structures were elucidated by the spectroscopic methods 1D and 2D NMR including COSY, HMQC, HMBC and NOE, and HREIMS. In our cytotoxicity assays, compound 5 showed cytotoxicity toward KB and KBv-200 cells with IC(50) of 1.40 and 2.58 microg/ml, respectively. In addition, the plausible biogenic relationship of compounds 1, 2, 3 and 4 is discussed.
我们报道了两种新天然产物，即1,4,5,6,7,9 - 六羟基 - 2 - 甲氧基 - 7 - 甲基 - 5β,9β,8aβ,6α,10aα - 六氢蒽 - 10(10aH) - 酮(1)和1,4,6 - 三羟基 - 2 - 甲氧基 - 7 - 甲基蒽 - 9,10 - 二酮(2)以及三种已知蒽醌的(1)H和(13)C NMR谱的明确归属。这些化合物均从采自中国南海的海洋内生真菌1403中分离得到。化合物3和4首次从该海洋真菌中分离得到。通过包括COSY、HMQC、HMBC和NOE的一维和二维NMR光谱方法以及高分辨电子轰击质谱(HREIMS)对其结构进行了阐明。在我们的细胞毒性试验中，化合物5对KB和KBv - 200细胞显示出细胞毒性，IC(50)分别为1.40和2.58微克/毫升。此外，还讨论了化合物1、2、3和4可能的生源关系。
