A 型产气荚膜梭菌回肠袢反应性菌株在磷酸盐缓冲液和牛肉汁中的热失活
Thermal inactivation of ileal loop-reactive Clostridium perfringens type A strains in phosphate buffer and beef gravy.
作者信息
Bradshaw J G, Peeler J T, Twedt R M
出版信息
Appl Environ Microbiol. 1977 Sep;34(3):280-4. doi: 10.1128/aem.34.3.280-284.1977.
Abstract
The thermal resistance of spore crops produced from each of two ileal loop-reactive strains of Clostridium perfringens type A was determined in two suspending vehicles consisting of 0.067 M (pH 7.0) phosphate buffer and a commercial beef gravy. D115.6 values obtained in buffer and enumerated after pretreatment with sodium ethylenediaminetetraacetate and recovery in plating medium containing lysozyme were two- to threefold greater than those obtained without this treatment. D115.6 values obtained with beef gravy were less than those obtained in buffer with or without lysozyme; however, the D98.9 and D104.4 values were 1.3 to 2 times greater than those obtained in buffer with lysozyme. The z values were within the ranges reported by previous investigators.
摘要
测定了两株回肠袢反应性A型产气荚膜梭菌产生的芽孢作物在两种悬浮介质中的热阻，这两种悬浮介质分别为0.067M（pH 7.0）磷酸盐缓冲液和市售牛肉汁。在缓冲液中获得的D115.6值，经乙二胺四乙酸钠预处理并在含有溶菌酶的平板培养基中复苏后计数，比未经此处理获得的值大两到三倍。用牛肉汁获得的D115.6值低于在有或没有溶菌酶的缓冲液中获得的值；然而，D98.9和D104.4值比在含有溶菌酶的缓冲液中获得的值大1.3至2倍。z值在先前研究者报告的范围内。
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