Department of Veterinary Population Medicine, 1333 Gortner Avenue, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN 55108, USA.
Vet J. 2011 Jan;187(1):139-41. doi: 10.1016/j.tvjl.2009.10.013. Epub 2009 Nov 14.
A PCR based method was developed for the identification of ceftiofur resistance genes (bla(CMY-2), bla(TEM-1), and ampC) in swine bacterial pathogens. Using this method, the ceftiofur resistant (n=76) and susceptible (n=45) strains of Bordetella bronchiseptica, Salmonella spp., Escherichia coli, and Pasteurella multocida were screened for the presence of these three genes. The resistant genes were detected in 70% (bla(TEM-1)), 68% (bla(CMY-2)) and 45% (ampC) of the resistant isolates and in 18% (bla(TEM-1)), 27% (bla(CMY-2)), and 36% (ampC) of the susceptible isolates. Results obtained in the present study showed widespread distribution of these three resistance genes in ceftiofur-resistant swine pathogens. It was also observed that more pathogens are acquiring these resistance genes.
本研究建立了一种基于 PCR 的方法，用于鉴定猪源病原菌中头孢噻呋耐药基因（bla(CMY-2)、bla(TEM-1)和 ampC）。使用该方法，对 76 株头孢噻呋耐药和 45 株头孢噻呋敏感的支气管败血波氏杆菌、沙门氏菌、大肠杆菌和多杀性巴氏杆菌进行了这三种基因的检测。耐药基因在 70%（bla(TEM-1)）、68%（bla(CMY-2)）和 45%（ampC）的耐药分离株中被检出，在 18%（bla(TEM-1)）、27%（bla(CMY-2)）和 36%（ampC）的敏感分离株中也被检出。本研究结果表明，这三种耐药基因在头孢噻呋耐药的猪源病原菌中广泛分布。同时还观察到，越来越多的病原菌正在获得这些耐药基因。
