猪细菌病原体中头孢噻呋耐药性的特征。
Characterisation of ceftiofur resistance in swine bacterial pathogens.
机构信息
Department of Veterinary Population Medicine, 1333 Gortner Avenue, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN 55108, USA.
出版信息
Vet J. 2011 Jan;187(1):139-41. doi: 10.1016/j.tvjl.2009.10.013. Epub 2009 Nov 14.
A PCR based method was developed for the identification of ceftiofur resistance genes (bla(CMY-2), bla(TEM-1), and ampC) in swine bacterial pathogens. Using this method, the ceftiofur resistant (n=76) and susceptible (n=45) strains of Bordetella bronchiseptica, Salmonella spp., Escherichia coli, and Pasteurella multocida were screened for the presence of these three genes. The resistant genes were detected in 70% (bla(TEM-1)), 68% (bla(CMY-2)) and 45% (ampC) of the resistant isolates and in 18% (bla(TEM-1)), 27% (bla(CMY-2)), and 36% (ampC) of the susceptible isolates. Results obtained in the present study showed widespread distribution of these three resistance genes in ceftiofur-resistant swine pathogens. It was also observed that more pathogens are acquiring these resistance genes.
本研究建立了一种基于 PCR 的方法，用于鉴定猪源病原菌中头孢噻呋耐药基因（bla(CMY-2)、bla(TEM-1)和 ampC）。使用该方法，对 76 株头孢噻呋耐药和 45 株头孢噻呋敏感的支气管败血波氏杆菌、沙门氏菌、大肠杆菌和多杀性巴氏杆菌进行了这三种基因的检测。耐药基因在 70%（bla(TEM-1)）、68%（bla(CMY-2)）和 45%（ampC）的耐药分离株中被检出，在 18%（bla(TEM-1)）、27%（bla(CMY-2)）和 36%（ampC）的敏感分离株中也被检出。本研究结果表明，这三种耐药基因在头孢噻呋耐药的猪源病原菌中广泛分布。同时还观察到，越来越多的病原菌正在获得这些耐药基因。
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