通过铜经由ACE 1因子进行转录控制，在酵母中铜、锌超氧化物歧化酶和金属硫蛋白基因表达共同调控的证据。

Evidence for co-regulation of Cu,Zn superoxide dismutase and metallothionein gene expression in yeast through transcriptional control by copper via the ACE 1 factor.

作者信息

Carri M T, Galiazzo F, Ciriolo M R, Rotilio G

机构信息

Department of Biology, University of Rome Tor Vergata, Italy.

出版信息

FEBS Lett. 1991 Jan 28;278(2):263-6. doi: 10.1016/0014-5793(91)80131-l.

DOI:10.1016/0014-5793(91)80131-l

PMID:1991520

Abstract

Saccharomyces cerevisiae mutant strain DTY26, lacking ACE1, the protein mediator for the induction of metallothionein gene expression, is unable to increase Cu,Zn superoxide dismutase mRNA in response to copper. In the wild-type strain DTY22 transcription of both Cu,Zn superoxide dismutase and metallothionein genes is induced by copper and silver, as expected on the basis of previous results indicating that ACE1 binds only Ag(I) besides Cu(I). We conclude that at the transcriptional level Cu,ZnSOD is co-regulated with metallothionein. Furthermore, structural similarities between the two promoters were found, which could explain the co-regulation effect and the quantitative differences in the response of the two genes to copper.

摘要

酿酒酵母突变株DTY26缺乏金属硫蛋白基因表达诱导的蛋白介导因子ACE1，无法响应铜增加铜锌超氧化物歧化酶mRNA的表达。在野生型菌株DTY22中，正如之前结果所预期的，即ACE1除了结合Cu(I)外还仅结合Ag(I)，铜和银均可诱导铜锌超氧化物歧化酶和金属硫蛋白基因的转录。我们得出结论，在转录水平上，铜锌超氧化物歧化酶与金属硫蛋白共同调控。此外，还发现了两个启动子之间的结构相似性，这可以解释共同调控效应以及两个基因对铜响应的定量差异。