铜对酿酒酵母过氧化氢酶基因表达的调控
Regulation of Saccharomyces cerevisiae catalase gene expression by copper.
作者信息
Lapinskas P, Ruis H, Culotta V
机构信息
Department of Environmental Health Sciences, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 21205.
出版信息
Curr Genet. 1993 Nov;24(5):388-93. doi: 10.1007/BF00351846.
Treatment of Saccharomyces cerevisiae cells with copper induces the activity of Cu/Zn superoxide dismutase (SOD) and catalase. To understand the level at which Cu regulates catalase, the expression of the S. cerevisiae CTA1 (encoding the peroxisomal catalase A) and CTT1 (encoding the cytosolic catalase T) genes was monitored as a function of Cu treatment. Copper was found to specifically induce transcription of CTT1, but not CTA1, mRNA. Moreover, genetic and biochemical studies demonstrate that this induction is independent of the ACE1 Cu trans-activator controlling the expression of yeast Cu/Zn SOD and metallothionein genes. Copper regulation of CTT1 thus appears to represent a novel metal regulatory pathway in S. cerevisiae cells.
用铜处理酿酒酵母细胞会诱导铜/锌超氧化物歧化酶（SOD）和过氧化氢酶的活性。为了解铜调节过氧化氢酶的水平，监测了酿酒酵母CTA1（编码过氧化物酶体过氧化氢酶A）和CTT1（编码胞质过氧化氢酶T）基因的表达随铜处理的变化情况。发现铜特异性地诱导CTT1 mRNA的转录，但不诱导CTA1 mRNA的转录。此外，遗传学和生物化学研究表明，这种诱导独立于控制酵母铜/锌SOD和金属硫蛋白基因表达的ACE1铜反式激活因子。因此，铜对CTT1的调节似乎代表了酿酒酵母细胞中一种新的金属调节途径。
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