Kruitwagen R F, Poels L G, Willemsen W N, de Ronde I J, Jap P H, Rolland R
Department of Obstetrics and Gynecology, St. Radboud University Hospital, Nijmegen, The Netherlands.
Fertil Steril. 1991 Feb;55(2):297-303. doi: 10.1016/s0015-0282(16)54119-3.
Peritoneal fluid (PF) was obtained during the early follicular phase in 24 women at laparoscopy as part of infertility investigation. The cells present in PF were pelleted and cultured. Developing endometrial epithelial cell colonies were identified in 19 women (79%). Identification of these cell colonies was facilitated using the monoclonal antibody BW 495/36 as specific marker. The number of endometrial epithelial cell colonies showed a large variation (1 to 200 or more PF sample). No significant distinction in incidence and number of cell colonies was found between women with minimal (n = 11) and without endometriosis (n = 12). A significant correlation with number of cell colonies was found in women with infertility and no mechanical and male infertility factors. These data indicate that retrograde transport of viable endometrial cells during menstruation occurs in most women with patent tubes. Implications of the results for the relation between retrograde menstruation, endometriosis, and infertility are discussed.
作为不孕症检查的一部分，在卵泡早期对24名女性进行腹腔镜检查时获取了腹腔液（PF）。将腹腔液中的细胞沉淀并进行培养。在19名女性（79%）中鉴定出了正在发育的子宫内膜上皮细胞集落。使用单克隆抗体BW 495/36作为特异性标志物有助于这些细胞集落的鉴定。子宫内膜上皮细胞集落的数量显示出很大的差异（每个PF样本为1至200个或更多）。在有轻微子宫内膜异位症（n = 11）和无子宫内膜异位症（n = 12）的女性之间，未发现细胞集落在发生率和数量上有显著差异。在无机械性和男性不育因素的不孕症女性中，发现细胞集落数量与之存在显著相关性。这些数据表明，在大多数输卵管通畅的女性中，月经期间有活力的子宫内膜细胞会发生逆行运输。讨论了这些结果对逆行月经、子宫内膜异位症和不孕症之间关系的影响。
