腹腔液中的上皮细胞——源自子宫内膜？

Epithelial cells in peritoneal fluid--of endometrial origin?

作者信息

van der Linden P J, Dunselman G A, de Goeij A F, van der Linden E P, Evers J L, Ramaekers F C

机构信息

Department of Obstetrics and Gynecology, Academisch Ziekenhuis Maastricht, The Netherlands.

出版信息

Am J Obstet Gynecol. 1995 Aug;173(2):566-70. doi: 10.1016/0002-9378(95)90283-x.

DOI:10.1016/0002-9378(95)90283-x

PMID:7544070

Abstract

OBJECTIVE

Our purpose was to examine the immunohistochemical properties of epithelial cells in peritoneal fluid and to compare the staining characteristics with cells of endometrium, menstrual effluent, peritoneum, and endometriotic lesions.

STUDY DESIGN

Samples of menstrual effluent, endometrium, and peritoneal fluid and biopsy specimens of endometriotic lesions and peritoneum from 16 patients were examined. Monoclonal antibodies against vimentin, cytokeratin 18 and 19, and the monoclonal antibody BW495/36, staining an epithelial marker present in endometrium and absent in peritoneal epithelium, were used.

RESULTS

All but one sample of menstrual effluent and peritoneal fluid cells stained positively with antibodies against vimentin and cytokeratin 18 and 19. BW495/36 stained 14 of 16 menstrual effluent samples and nine of 16 peritoneal fluid cell samples. Endometriotic specimens showed staining with all markers. No major differences in staining properties were observed in menstrual effluent, endometrium, and peritoneal fluid cells between patients with or without endometriosis.

CONCLUSION

These results support the contention of transport of menstrual detritus to the peritoneal cavity in women with patent fallopian tubes.

摘要

目的

我们的目的是研究腹腔液中上皮细胞的免疫组织化学特性，并将染色特征与子宫内膜、月经流出物、腹膜和子宫内膜异位症病变的细胞进行比较。

研究设计

检查了16例患者的月经流出物、子宫内膜和腹腔液样本以及子宫内膜异位症病变和腹膜的活检标本。使用了针对波形蛋白、细胞角蛋白18和19的单克隆抗体，以及单克隆抗体BW495/36，该抗体可染色子宫内膜中存在而腹膜上皮中不存在的一种上皮标志物。

结果

除一份月经流出物和腹腔液细胞样本外，所有样本对波形蛋白以及细胞角蛋白18和19的抗体染色均呈阳性。BW495/36对16份月经流出物样本中的14份和16份腹腔液细胞样本中的9份进行了染色。子宫内膜异位症标本对所有标志物均呈染色阳性。在有或没有子宫内膜异位症的患者之间，月经流出物、子宫内膜和腹腔液细胞的染色特性未观察到重大差异。