Siqueira A B S, Gomes B S, Cambuim I, Maia R, Abreu S, Souza-Motta C M, de Queiroz L A, Porto A L F
Department of Mycology, Federal University of Pernambuco (UFPE), Recife-PE, Brazil.
Lett Appl Microbiol. 2009 Jan;48(1):90-6. doi: 10.1111/j.1472-765X.2008.02494.x. Epub 2008 Nov 14.
The in vitro antifungal activity of Brazilian green and red propolis was tested against different species of Trichophyton.
The antifungal activity of the Brazilian aqueous and alcoholic extracts of the green propolis and the alcoholic extract of red propolis was observed against Trichophyton rubrum, Trichophyton tonsurans and Trichophyton mentagrohytes samples, using as controls itraconazole and terbinafine. The minimal inhibitory concentration was determined following the microdilution method indicated by the 'Clinical and Laboratory Standards Institute'. The minimal fungicide concentration was determined by the absence of growth in liquid sabouraud culture medium. The data obtained showed that the green propolis alcoholic extract's antifungal activity was from 64 to 1024 microg ml(-1), whereas the red propolis alcoholic extract was from 8 to 1024 microg ml(-1).
The antifungal activity of the red propolis alcoholic extract was more efficient than the green propolis alcoholic extract for all three species studied. The T. rubrum samples were shown to be more sensitive to the antifungal activity of the alcoholic extracts of the propolis.
The antifungal potential of the alcoholic extracts of green and red propolis demonstrated suggest an applicable potential as an alternative treatment for dermatophytosis caused by these species.
测试巴西绿蜂胶和红蜂胶对不同毛癣菌属菌种的体外抗真菌活性。
观察巴西绿蜂胶的水提取物和醇提取物以及红蜂胶醇提取物对红色毛癣菌、断发毛癣菌和须癣毛癣菌样本的抗真菌活性，以伊曲康唑和特比萘芬作为对照。按照“临床和实验室标准协会”指示的微量稀释法测定最低抑菌浓度。通过液体沙氏培养基中无生长来确定最低杀菌浓度。所获得的数据表明，绿蜂胶醇提取物的抗真菌活性为64至1024微克/毫升，而红蜂胶醇提取物为8至1024微克/毫升。
对于所研究的所有三种菌种，红蜂胶醇提取物的抗真菌活性比绿蜂胶醇提取物更有效。红色毛癣菌样本对蜂胶醇提取物的抗真菌活性表现出更高的敏感性。
所证明的绿蜂胶和红蜂胶醇提取物的抗真菌潜力表明，其作为这些菌种引起的皮肤癣菌病的替代治疗方法具有应用潜力。
