Vera Nancy, Solorzano Eliana, Ordoñez Roxana, Maldonado Luis, Bedascarrasbure Enrique, Isla María I
Facultad de Bioquímica, Química y Farmacia, Ayacucho 471, Universidad Nacional de Tucumán, 4000 San Miguel de Tucumán, Argentina.
Nat Prod Commun. 2011 Jun;6(6):823-7.
This paper reveals, for the first time, the functional properties of propolis from an extreme region of Argentine (El Rincón, Province of Catamarca, Argentina), as well as the isolation and identification of bioactive compounds. The antioxidant activity was determined by the ABTS method and beta-carotene bleaching. The antibacterial activity was determined on methicillin resistant Staphylococcus aureus (MRSA) by the microdilution method and bioautographic assays. Twelve compounds were isolated and identified by NMR spectroscopy. The main bioactive compounds were 2',4'-dihydroxy-3'-methoxychalcone (3), 2',4'-dihydroxychalcone (9), 2',4',4-trihydroxy-6'- methoxychalcone (8), 5-hydroxy-4',7-dimethoxyflavone (4), 4',5-dihydroxy-3,7,8-trimethoxyflavone (10) and 7-hydroxy- 5,8-dimethoxyflavone (11). All compounds were active against clinical isolates (MIC50 10 microg/mL) and displayed antioxidant activity (SC50 values of 20 microg/mL). The MIC and SC50 values of the isolated compounds were lower than those obtained with crude propolis extracts, chloroform sub-extracts and isolated fractions.
本文首次揭示了来自阿根廷一个极端地区（阿根廷卡塔马卡省埃尔林孔）的蜂胶的功能特性，以及生物活性化合物的分离和鉴定。通过ABTS法和β-胡萝卜素漂白法测定抗氧化活性。通过微量稀释法和生物自显影法测定对耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌活性。通过核磁共振光谱法分离和鉴定了12种化合物。主要生物活性化合物为2',4'-二羟基-3'-甲氧基查耳酮（3）、2',4'-二羟基查耳酮（9）、2',4',4-三羟基-6'-甲氧基查耳酮（8）、5-羟基-4',7-二甲氧基黄酮（4）、4',5-二羟基-3,7,8-三甲氧基黄酮（10）和7-羟基-5,8-二甲氧基黄酮（11）。所有化合物对临床分离株均有活性（MIC50为10微克/毫升）并显示出抗氧化活性（SC50值为20微克/毫升）。分离得到的化合物的MIC和SC50值低于用粗蜂胶提取物、氯仿亚提取物和分离组分获得的值。
