Chung Tzu-Yun, Peplow Philip V, Baxter G David
Department of Anatomy & Structural Biology, University of Otago, Dunedin, New Zealand.
Photomed Laser Surg. 2010 Apr;28(2):251-61. doi: 10.1089/pho.2009.2493.
The aim of this investigation was to compare the healing of laser-irradiated and non-irradiated wounds covered by an occlusive dressing in mice.
Many previous studies of the effects of laser irradiation of experimental wounds in mice and rats did not cover the wounds so that healing occurred mainly by contraction. Healing of covered wounds is slower and mimics more closely wound healing in humans.
Forty-seven diabetic and twenty non-diabetic mice were used. A single wound (5 mm diameter) was created on the left flank of each animal and covered by Tegaderm HP dressing (Day 1). Wounds were irradiated (660 nm) for 20 s using a lower power (18 mW) or higher power (80 mW) laser starting immediately post-wounding for seven consecutive days (0.36 or 1.6 J/day); untreated wounds served as controls. Animals were euthanized on Day 8, 10, or 14. Wound specimens were cut and stained using haematoxylin and eosin, and picrosirius red, and examined by microscopy.
Results confirmed that wound healing was impaired in diabetic mice. Analysis of the data demonstrated that Tegaderm HP dressing had retarded contraction (splinted the wounds) in a large proportion of diabetic mice and, to a lesser extent, in non-diabetic mice. Healing of splinted wounds was delayed compared to unsplinted wounds, but laser irradiation (1.6 J/day, 7 days) stimulated healing by re-epithelization and granulation tissue formation.
These are the first findings of laser-mediated stimulation of healing in splinted wounds. Further studies are needed to assess the effects of different constellation sets of laser parameters in this wound model.
本研究旨在比较用封闭敷料覆盖的激光照射伤口与未照射伤口在小鼠体内的愈合情况。
此前许多关于激光照射对小鼠和大鼠实验性伤口影响的研究未对伤口进行覆盖，因此愈合主要通过收缩发生。覆盖伤口的愈合较慢，更接近人类伤口愈合情况。
使用47只糖尿病小鼠和20只非糖尿病小鼠。在每只动物的左侧胁腹处制造一个单一伤口（直径5毫米），并在第1天用德湿欣HP敷料覆盖。从受伤后立即开始，使用低功率（18毫瓦）或高功率（80毫瓦）激光对伤口进行照射20秒，连续7天（0.36或1.6焦耳/天）；未处理的伤口作为对照。在第8、10或14天对动物实施安乐死。将伤口标本进行苏木精-伊红染色和天狼星红染色，然后通过显微镜检查。
结果证实糖尿病小鼠的伤口愈合受损。数据分析表明，德湿欣HP敷料在很大比例的糖尿病小鼠中延缓了收缩（固定了伤口），在非糖尿病小鼠中程度较轻。与未固定的伤口相比，固定伤口的愈合延迟，但激光照射（1.6焦耳/天，7天）通过再上皮化和肉芽组织形成促进了愈合。
这些是关于激光介导刺激固定伤口愈合的首次研究结果。需要进一步研究来评估该伤口模型中不同激光参数组合的效果。
