Peplow Philip V, Chung Tzu-Yun, Baxter G David
Department of Anatomy and Structural Biology, University of Otago, Dunedin, New Zealand.
Lasers Surg Med. 2012 Jan;44(1):26-9. doi: 10.1002/lsm.21133. Epub 2011 Nov 22.
We have used a 660-nm laser diode in genetic diabetic mice to stimulate the healing of wounds covered with a Tegaderm HP dressing that causes a retardation of contraction (splinted wounds). It is possible that the stimulation of healing could be due to possible diabetes-modifying properties of laser light. This has been examined by using the 660 nm laser to irradiate non-wounded diabetic mice with the same dose and at same location as for wounded diabetic mice.
Twenty-two diabetic mice were used and divided into two equal groups. Body weight and water intake of mice were measured daily for 7 days prior to the start of treatment (Day 0). The left flank of mice in the experimental group was irradiated with 660 nm laser, 100 mW, 20 seconds/day, 7 days; for mice in the control group, the left flank was sham-treated with the laser power supply not switched on. Body weight and water intake of mice were measured to Day 14. On Day 14, the mice were fasted for 4 hours, anaesthetized with sodium pentobarbitone (i.p.) and blood collected by cardiac puncture into heparinized tubes. The plasma was assayed for glucose and glycated hemoglobin A1c.
There were no significant differences in body weight and water intake over 22 days between mice in the experimental group and control group. On day 14, the mean blood plasma glucose level was not significantly different between the two groups; glycated hemoglobin A1c was not detected in the samples.
Irradiation of the left flank in diabetic mice with 660 nm laser system does not have a significant hypoglycemic effect, and the laser-stimulated healing of wounds in diabetic mice is due to cellular and biochemical changes in the immediate wound environment.
我们已在遗传性糖尿病小鼠中使用660纳米激光二极管，以刺激覆盖有泰德 Derm HP 敷料（该敷料会导致收缩延迟，即夹板固定伤口）的伤口愈合。愈合刺激可能是由于激光光可能具有的糖尿病改善特性。通过使用660纳米激光以与受伤糖尿病小鼠相同的剂量和相同位置照射未受伤的糖尿病小鼠来对此进行了研究。
使用22只糖尿病小鼠并将其分为两组。在治疗开始前7天（第0天）每天测量小鼠的体重和饮水量，持续7天。实验组小鼠的左侧胁腹用660纳米激光照射，功率100毫瓦，每天照射20秒，共照射7天；对照组小鼠的左侧胁腹进行假处理，即不打开激光电源。测量小鼠的体重和饮水量直至第14天。在第14天，小鼠禁食4小时，腹腔注射戊巴比妥钠麻醉后，通过心脏穿刺将血液收集到肝素化管中。测定血浆中的葡萄糖和糖化血红蛋白A1c。
实验组和对照组小鼠在22天内的体重和饮水量没有显著差异。在第14天，两组之间的平均血浆葡萄糖水平没有显著差异；样本中未检测到糖化血红蛋白A1c。
用660纳米激光系统照射糖尿病小鼠的左侧胁腹没有显著的降血糖作用，糖尿病小鼠伤口的激光刺激愈合是由于伤口局部环境中的细胞和生化变化。
