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J Am Chem Soc. 2009 Nov 25;131(46):16660-2. doi: 10.1021/ja9046944.
This paper describes the construction of a combinatorial artificial receptor array (CARA) and the application of the array to differentiation of proteins based on their binding patterns. Microarrays displaying 5035 unique binding environments were prepared using a library of 19 small molecule building blocks. Each building block was equipped with a carboxylic acid handle, allowing mixtures of the building blocks to be spotted onto the surface of an amine functionalized glass slide for covalent immobilization as subunits of the binding environments. This strategy employs the microarray surface as the receptor synthesis platform, which allows for flexibility in array preparation and agility in application. An advantage of the CARA strategy is the enormous flexibility it enables in the construction of alternate microarray configurations, which facilitates rapid access to the breadth and depth of binding space. Four fluorescently labeled proteins, ubiquitin, myoglobin, alpha-1-acid glycoprotein and lysozyme, were incubated with the arrays to demonstrate the reproducibility of binding and the level of differentiation that can be achieved. The binding environments are stable, scalable, and adaptable to other formats.
本文描述了一种组合人工受体阵列（CARA）的构建及其在基于结合模式区分蛋白质方面的应用。该阵列使用了 19 个小分子构建基块的库来制备显示 5035 个独特结合环境的微阵列。每个构建基块都配备了一个羧酸处理手柄，允许将构建基块的混合物点样到胺功能化玻璃载玻片的表面上，作为结合环境的亚单位进行共价固定。这种策略将微阵列表面用作受体合成平台，这使得阵列制备具有灵活性，并且在应用方面具有敏捷性。CARA 策略的一个优势是它在构建替代微阵列配置方面具有极大的灵活性，这有助于快速访问结合空间的广度和深度。四种荧光标记的蛋白质，即泛素、肌红蛋白、α-1-酸性糖蛋白和溶菌酶，与阵列孵育，以证明结合的重现性和可以实现的区分水平。结合环境稳定、可扩展且适用于其他格式。
