不同解离程序后肿瘤细胞表面蛋白的放射性碘化研究。
Radioiodination studies of tumour cell-surface proteins after different disaggregation procedures.
作者信息
Guy D, Latner A L, Turner G A
出版信息
Br J Cancer. 1977 Aug;36(2):166-72. doi: 10.1038/bjc.1977.173.
Abstract
The surface of single cells isolated from solid tumours by either a mechanical or an enzymatic method have been compared, using lactoperoxidase-catalyzed radioiodination of the tyrosine-containing proteins. Qualitatively, the patterns of surface labelling were similar, and duplicate experiments indicated that each method of isolation gave reproducible results. Analysis of incorporated label into 4 defined sections of the electrophoretic pattern illustrated quantitative differences. When the cells were isolated mechanically, the incorporation into low-mol.- wt. components was considerably reduced, whereas that into the high-mol.-wt. components was unaffected. Treatment of enzymatically isolated cells with trypsin also reduced incorporation into low-mol.-wt components.
摘要
通过机械或酶法从实体瘤中分离出的单细胞表面，已使用乳过氧化物酶催化的含酪氨酸蛋白放射性碘化进行了比较。定性地说，表面标记模式相似，重复实验表明每种分离方法都能给出可重复的结果。对电泳图谱4个定义部分中掺入的标记物进行分析，显示出定量差异。当细胞通过机械方法分离时，低分子量组分中的掺入量显著减少，而高分子量组分中的掺入量不受影响。用胰蛋白酶处理酶法分离的细胞也会减少低分子量组分中的掺入量。
相似文献
1
Radioiodination studies of tumour cell-surface proteins after different disaggregation procedures.不同解离程序后肿瘤细胞表面蛋白的放射性碘化研究。
Br J Cancer. 1977 Aug;36(2):166-72. doi: 10.1038/bjc.1977.173.
2
Surface protein distributions in cells isolated from solid tumours and their metastases.实体瘤及其转移灶分离出的细胞中的表面蛋白分布
Br J Cancer. 1979 Oct;40(4):634-40. doi: 10.1038/bjc.1979.229.
3
Differences in surface expression of WGA-binding proteins of cells from a lymphosarcoma and its liver metastases.淋巴肉瘤及其肝转移灶细胞中WGA结合蛋白的表面表达差异。
Br J Cancer. 1984 Feb;49(2):181-91. doi: 10.1038/bjc.1984.30.
4
Lactoperoxidase-catalysed iodination of surface proteins on human melanoma cells.乳过氧化物酶催化的人黑色素瘤细胞表面蛋白碘化作用。
Br J Cancer. 1978 Jul;38(1):114-21. doi: 10.1038/bjc.1978.170.
5
Comparison of the surface glycoprotein components in the isolated cells of hamster melanotic and amelanotic melanomas.
Arch Dermatol Res (1975). 1976 Aug 27;256(2):197-203. doi: 10.1007/BF00567365.
6
Plasma membrane of a murine T cell lymphoma: surface labelling, membrane isolation, separation of membrane proteins and distribution of surface label amongst these proteins.
Aust J Exp Biol Med Sci. 1976 Aug;54(4):303-16. doi: 10.1038/icb.1976.31.
7
Proteins of the kidney microvillar membrane. Asymmetric labelling of the membrane by lactoperoxidase-catalysed radioiodination and by photolysis of 3,5-di[125I]iodo-4-azidobenzenesulphonate.肾微绒毛膜蛋白。通过乳过氧化物酶催化的放射性碘化以及3,5-二[125I]碘-4-叠氮基苯磺酸盐的光解对膜进行不对称标记。
Biochem J. 1980 Apr 1;187(1):31-44. doi: 10.1042/bj1870031.
8
The comparison of membrane proteins of normal and neoplastic cells by isoelectric focusing.通过等电聚焦对正常细胞和肿瘤细胞的膜蛋白进行比较。
Biochem Soc Trans. 1976;4(4):675-7. doi: 10.1042/bst0040675.
9
Increased release of tumour cells by collagenase at acid pH: a possible mechanism for metastasis.酸性pH条件下胶原酶使肿瘤细胞释放增加：转移的一种可能机制。
Experientia. 1979 Dec 15;35(12):1657-8. doi: 10.1007/BF01953252.
引用本文的文献
1
Possible surface protein markers for breast cancer.乳腺癌可能的表面蛋白标志物。
Br J Cancer. 1981 Jan;43(1):118-21. doi: 10.1038/bjc.1981.19.
2
Surface properties of cells isolated from non-metastasizing and metastasizing hamster lymphosarcomas.从非转移性和转移性仓鼠淋巴肉瘤中分离出的细胞的表面特性。
Br J Cancer. 1980 Dec;42(6):915-21. doi: 10.1038/bjc.1980.340.
3
Differences in surface expression of WGA-binding proteins of cells from a lymphosarcoma and its liver metastases.淋巴肉瘤及其肝转移灶细胞中WGA结合蛋白的表面表达差异。
Br J Cancer. 1984 Feb;49(2):181-91. doi: 10.1038/bjc.1984.30.
4
Antigenic differences between a primary hamster lymphosarcoma and its liver metastases.原发性仓鼠淋巴肉瘤与其肝转移瘤之间的抗原差异。
Experientia. 1982 May 15;38(5):610-1. doi: 10.1007/BF02327078.
5
The growth and metastasis of four commonly used tumour lines implanted into eight different sites: evidence for site and tumour effects.
Clin Exp Metastasis. 1988 May-Jun;6(3):233-44. doi: 10.1007/BF01782483.
6
Surface protein distributions in cells isolated from solid tumours and their metastases.实体瘤及其转移灶分离出的细胞中的表面蛋白分布
Br J Cancer. 1979 Oct;40(4):634-40. doi: 10.1038/bjc.1979.229.
7
Increased release of tumour cells by collagenase at acid pH: a possible mechanism for metastasis.酸性pH条件下胶原酶使肿瘤细胞释放增加：转移的一种可能机制。
Experientia. 1979 Dec 15;35(12):1657-8. doi: 10.1007/BF01953252.
本文引用的文献
1
A new method for the preparation of liver cell suspensions.一种制备肝细胞悬液的新方法。
Exp Cell Res. 1962 Sep;27:453-67. doi: 10.1016/0014-4827(62)90011-3.
2
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
3
Separation of viable from non-viable tumor cells using Ficoll-Hypaque density solution.
Immunol Commun. 1973;2(5):463-72. doi: 10.3109/08820137309022818.
4
Alteration of cell-surface proteins by viral transformation and by proteolysis.病毒转化和蛋白水解对细胞表面蛋白的改变。
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3170-4. doi: 10.1073/pnas.70.11.3170.
5
Improved method for preparation of single-cell suspensions from mammary glands of adult virgin mouse.
Exp Cell Res. 1970 Aug;61(2):451-4. doi: 10.1016/0014-4827(70)90470-2.
6
The binding of sodium dodecyl sulphate to various proteins.十二烷基硫酸钠与各种蛋白质的结合。
Biochem J. 1968 Oct;109(5):825-30. doi: 10.1042/bj1090825.
7
An evaluation of techniques for labelling the surface proteins of cultured mammalian cells.
Biochim Biophys Acta. 1975 Jan 28;375(2):249-67. doi: 10.1016/0005-2736(75)90193-5.
8
Cell surface proteins and malignant transformation.细胞表面蛋白与恶性转化
Biochim Biophys Acta. 1976 Apr 30;458(1):73-107. doi: 10.1016/0304-419x(76)90015-9.
Zotero 插件