Unit International Health, Epidemiology and Social Medicine, Antwerp University, Belgium.
Malar J. 2009 Nov 18;8:258. doi: 10.1186/1475-2875-8-258.
HIV-1 infection affects malaria humeral immunity during pregnancy, but data for non-pregnant adults are lacking. This study reports the impact of HIV-1 infection and other variables on the level of malaria humeral immunity in adults with clinical malaria and whether humeral immune suppression was a risk factor for treatment failure.
Sera of 224 HIV-1 infected and 115 uninfected adults were compared for IgG to merozoite antigens AMA-1 and MSP2 (3D7 and FC27 types) determined by ELISA, and for IgG to the Variant Surface Antigens (VSA) of three different parasite line E8B, A4 and HCD6 determined by flow cytometry.
Compared to HIV-1 uninfected adults, AMA-1 IgG was lower in HIV-1 infected (P = 0.02) and associated with low CD4 count AMA-1 IgG (P = 0.003). Low IgG to all three merozoite antigens was associated with less anemia (P = 0.03). High parasite load was associated with low MSP2 IgG 3D7 and FC27 types (P = 0.02 and P = 0.08). Antibody levels to VSA did not differ between HIV-1 infected and uninfected adults. However, low VSA IgGs were associated with high parasite load (P <or= 0.002 for each parasite line) and with treatment failure (P <or= 0.04 for each parasite line).
HIV-1 affects humeral responses to AMA-1, but seems to marginally or not affect humeral responses to other merozoite antigens and VSAs. The latter were important for controlling parasite density and predict treatment outcome.
HIV-1 感染会影响妊娠期间疟疾的体液免疫，但缺乏非妊娠成人的数据。本研究报告了 HIV-1 感染和其他变量对患有临床疟疾的成年人体内疟疾体液免疫水平的影响，以及体液免疫抑制是否是治疗失败的一个危险因素。
通过 ELISA 比较了 224 名 HIV-1 感染和 115 名未感染的成年人血清中针对裂殖子抗原 AMA-1 和 MSP2（3D7 和 FC27 型）的 IgG 水平，通过流式细胞术比较了针对三种不同寄生虫系 E8B、A4 和 HCD6 的三种不同寄生虫系 E8B、A4 和 HCD6 的 VSA 的 IgG 水平。
与 HIV-1 未感染的成年人相比，HIV-1 感染的成年人 AMA-1 IgG 水平较低（P = 0.02），且与低 CD4 计数 AMA-1 IgG 相关（P = 0.003）。所有三种裂殖子抗原的 IgG 水平较低与贫血程度较轻相关（P = 0.03）。高寄生虫负荷与 MSP2 IgG 3D7 和 FC27 型较低相关（P = 0.02 和 P = 0.08）。HIV-1 感染和未感染的成年人之间 VSA 的抗体水平没有差异。然而，低 VSA IgG 与高寄生虫负荷（每个寄生虫系均为 P < 0.002）和治疗失败（每个寄生虫系均为 P < 0.04）相关。
HIV-1 影响 AMA-1 的体液反应，但似乎对其他裂殖子抗原和 VSA 的体液反应影响较小或没有影响。后者对控制寄生虫密度和预测治疗结果很重要。
