消除肺炎球菌抗体Luminex检测中的假阳性结果。
Elimination of false-positive results in a luminex assay for pneumococcal antibodies.
作者信息
Pickering Jerry W, Larson Matthew T, Martins Thomas B, Copple Susan S, Hill Harry R
机构信息
ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA.
出版信息
Clin Vaccine Immunol. 2010 Jan;17(1):185-9. doi: 10.1128/CVI.00329-09. Epub 2009 Nov 18.
Abstract
In our 14-valent Luminex assay for pneumococcal antibodies, we identified two groups of sera that caused false-positive results. The first group bound nonspecifically to the Luminex microspheres. The second group reacted specifically with bovine serum albumin (BSA). We describe here methods that eliminated the false-positive reactivity of both groups.
摘要
在我们用于检测肺炎球菌抗体的14价Luminex检测中，我们鉴定出两组导致假阳性结果的血清。第一组血清与Luminex微球发生非特异性结合。第二组血清与牛血清白蛋白（BSA）发生特异性反应。我们在此描述了消除两组血清假阳性反应性的方法。
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10
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