Kim Mi Sun, Shin Dong Hae
Ewha Womans University, Seoul, Republic of Korea.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Nov 1;65(Pt 11):1110-2. doi: 10.1107/S174430910903259X. Epub 2009 Oct 30.
Sedoheptulose-7-phosphate isomerase (GmhA) converts d-sedoheptulose 7-phosphate to d,d-heptose 7-phosphate. This is the first step in the biosynthesis pathway of NDP-heptose, which is responsible for the pleiotropic phenotype. This biosynthesis pathway is the target of inhibitors to increase the membrane permeability of Gram-negative pathogens or of adjuvants working synergistically with known antibiotics. Burkholderia pseudomallei is the causative agent of melioidosis, a seriously invasive disease in animals and humans in tropical and subtropical areas. GmhA from B. pseudomallei is one of the targets of antibiotic adjuvants for melioidosis. In this study, GmhA has been cloned, expressed, purified and crystallized. Synchrotron X-ray data were also collected to 1.9 angstrom resolution. The crystal belonged to the primitive orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 61.3, b = 84.2, c = 142.3 angstrom. A full structural determination is under way in order to provide insights into the structure- function relationships of this protein.
景天庚酮糖-7-磷酸异构酶（GmhA）将D-景天庚酮糖7-磷酸转化为D，D-庚糖7-磷酸。这是NDP-庚糖生物合成途径的第一步，该途径导致了多效性表型。这条生物合成途径是增加革兰氏阴性病原体膜通透性的抑制剂或与已知抗生素协同作用的佐剂的作用靶点。类鼻疽杆菌是类鼻疽病的病原体，类鼻疽病是热带和亚热带地区动物和人类的一种严重侵袭性疾病。来自类鼻疽杆菌的GmhA是类鼻疽病抗生素佐剂的靶点之一。在本研究中，GmhA已被克隆、表达、纯化并结晶。同步辐射X射线数据也已收集至1.9埃分辨率。该晶体属于原始正交空间群P2(1)2(1)2(1)，晶胞参数a = 61.3，b = 84.2，c = 142.3埃。目前正在进行完整的结构测定，以便深入了解该蛋白质的结构-功能关系。
