Kim Mi Sun, Lim Areum, Yang Seung Won, Lee Daeun, Park Jimin, Shin Dong Hae
The Center for Cell Signaling and Drug Discovery Research, College of Pharmacy, Division of Life and Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, Republic of Korea.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1554-6. doi: 10.1107/S1744309112044375. Epub 2012 Nov 28.
TktA is the most critical enzyme in the nonoxidative pentose phosphate pathway. It catalyzes the conversion of xylulose 5-phosphate and ribose 5-phosphate into sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate, and its products are used in the biosynthesis of acetyl-CoA, aromatic amino acids, nucleic acids and ADP-L-glycero-β-D-manno-heptose. TktA also has an unexpected role in chromosome structure that is independent of its metabolic responsibilities. Therefore, it is a new potent antibiotic target. In this study, TktA from Burkholderia pseudomallei has been cloned, expressed, purified and crystallized. Synchrotron X-ray data were also collected to 2.0 Å resolution. The crystal belonged to the monoclinic space group C2, with unit-cell parameters a=146.2, b=74.6, c=61.6 Å, β=113.0°. A full structural determination is under way in order to provide insight into the structure-function relationship of this protein.
转酮醇酶A（TktA）是非氧化戊糖磷酸途径中最关键的酶。它催化5-磷酸木酮糖和5-磷酸核糖转化为7-磷酸景天庚酮糖和3-磷酸甘油醛，其产物用于乙酰辅酶A、芳香族氨基酸、核酸和ADP-L-甘油-β-D-甘露庚糖的生物合成。TktA在染色体结构中还具有一个与其代谢功能无关的意外作用。因此，它是一个新的有效抗生素靶点。在本研究中，已对来自类鼻疽伯克霍尔德菌的TktA进行了克隆、表达、纯化和结晶。还收集了分辨率为2.0 Å的同步辐射X射线数据。该晶体属于单斜空间群C2，晶胞参数为a = 146.2、b = 74.6、c = 61.6 Å，β = 113.0°。目前正在进行完整的结构测定，以便深入了解该蛋白质的结构-功能关系。
