Kim Mi Sun, Shin Dong Hae
College of Pharmacy, Division of Life and Pharmaceutical Sciences and Center for Cell Signaling and Drug Discovery Research, Ewha Womans University, Seoul 120-750, Republic of Korea.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Feb 1;66(Pt 2):160-2. doi: 10.1107/S1744309109042614. Epub 2010 Jan 27.
D,D-Heptose-1,7-bisphosphate phosphatase (GmhB), which is involved in the third step of the NDP-heptose biosynthesis pathway, converts D,D-heptose-1,7-bisphosphate to D,D-heptose-1-phosphate. This biosynthesis pathway is a target for new antibiotics or antibiotic adjuvants for Gram-negative pathogens. Burkholderia thailandensis is a useful surrogate organism for studying the pathogenicity of melioidosis owing to its extensive genomic similarity to B. pseudomallei. Melioidosis caused by B. pseudomallei is a serious invasive disease of animals and humans in tropical and subtropical areas. In this study, GmhB has been cloned, expressed, purified and crystallized. X-ray data have also been collected to 2.50 A resolution using synchrotron radiation. The crystal belonged to space group P6, with unit-cell parameters a = 243.2, b = 243.2, c = 41.1 A.
D,D-庚糖-1,7-二磷酸磷酸酶（GmhB）参与NDP-庚糖生物合成途径的第三步，将D,D-庚糖-1,7-二磷酸转化为D,D-庚糖-1-磷酸。这条生物合成途径是新型抗生素或革兰氏阴性病原体抗生素佐剂的作用靶点。泰国伯克霍尔德菌由于其与类鼻疽伯克霍尔德菌在基因组上具有广泛的相似性，是研究类鼻疽病致病性的有用替代生物。类鼻疽伯克霍尔德菌引起的类鼻疽病是热带和亚热带地区动物和人类的一种严重侵袭性疾病。在本研究中，GmhB已被克隆、表达、纯化并结晶。还利用同步辐射收集了分辨率为2.50 Å的X射线数据。晶体属于空间群P6，晶胞参数a = 243.2，b = 243.2，c = 41.1 Å。
