钙离子诱导形成了两个不同的 II 组内含子分子亚群。
Ca2+ induces the formation of two distinct subpopulations of group II intron molecules.
机构信息
Institute of Inorganic Chemistry, University of Zürich, Winterthurerstrasse 190, 8057 Zürich, Switzerland.
出版信息
Angew Chem Int Ed Engl. 2009;48(51):9739-42. doi: 10.1002/anie.200903809.
Abstract
The folding pathway of the .ai5γ derived group II intron ribozyme D135 is highly specific to the correct M cofactor. Upon partial replacement of Mg with Ca, the molecules split into two distinct static subpopulations that are not interchangeable. Type 2 molecules thereby form a compact but misfolded state.
摘要
.D135 衍生的 II 类内含子核酶的折叠途径对正确的 M 辅因子具有高度特异性。用 Ca 部分取代 Mg 后，分子分裂成两个不同的静态亚群，它们不能互换。因此，2 型分子形成紧凑但错误折叠的状态。
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1
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Methods. 2009 Oct;49(2):112-7. doi: 10.1016/j.ymeth.2009.04.017. Epub 2009 May 4.
2
Single-molecule studies of group II intron ribozymes.
Proc Natl Acad Sci U S A. 2008 Sep 16;105(37):13853-8. doi: 10.1073/pnas.0804034105. Epub 2008 Sep 4.
3
Metal ion specificities for folding and cleavage activity in the Schistosoma hammerhead ribozyme.
RNA. 2008 Oct;14(10):2212-22. doi: 10.1261/rna.1010808. Epub 2008 Aug 28.
4
Divalent metal ions tune the self-splicing reaction of the yeast mitochondrial group II intron Sc.ai5gamma.
J Biol Inorg Chem. 2008 Aug;13(6):1025-36. doi: 10.1007/s00775-008-0390-7. Epub 2008 Jun 5.
5
Group II introns: structure, folding and splicing mechanism.
Biol Chem. 2007 Jul;388(7):665-78. doi: 10.1515/BC.2007.090.
6
Alternative roles for metal ions in enzyme catalysis and the implications for ribozyme chemistry.
Chem Rev. 2007 Jan;107(1):97-113. doi: 10.1021/cr0502605.
7
Extraordinary rates of transition metal ion-mediated ribozyme catalysis.
RNA. 2006 Oct;12(10):1846-52. doi: 10.1261/rna.128906. Epub 2006 Aug 15.
8
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Methods Mol Biol. 2006;335:289-310. doi: 10.1385/1-59745-069-3:289.
9
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10
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