Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.
Vaccine. 2010 Jan 22;28(4):940-9. doi: 10.1016/j.vaccine.2009.10.146. Epub 2009 Dec 3.
The F(0)F(1) ATPase plays a central role in both the generation of ATP and the utilisation of ATP for cellular processes such as rotation of bacterial flagella. We have deleted the entire operon encoding the F(0)F(1) ATPase, as well as genes encoding individual F(0) or F(1) subunits, in Salmonella enteric serovar Typhimurium. These mutants were attenuated for virulence, as assessed by bacterial counts in the livers and spleens of intravenously infected mice. The attenuated in vivo growth of the entire atp operon mutant was complemented by the insertion of the atp operon into the malXY pseudogene region. Following clearance of the attenuated mutants from the organs, mice were protected against challenge with the virulent wild type parent strain. We have shown that the F(0)F(1) ATPase is important for bacterial growth in vivo and that atp mutants are effective live attenuated vaccines against Salmonella infection.
F(0)F(1)ATP 合酶在 ATP 的生成和利用中都起着核心作用，可用于细胞过程，如细菌鞭毛的旋转。我们已经删除了编码 F(0)F(1)ATP 合酶的整个操纵子，以及编码单个 F(0)或 F(1)亚基的基因，在肠炎沙门氏菌血清型 Typhimurium 中。这些突变体的毒力减弱，通过静脉感染小鼠的肝脏和脾脏中的细菌计数来评估。整个 atp 操纵子突变体的体内生长减弱，通过将 atp 操纵子插入 malXY 假基因区域得到了补充。减弱的突变体从器官中清除后，小鼠对毒力野生型亲本菌株的攻击具有保护作用。我们已经表明，F(0)F(1)ATP 合酶对于体内细菌生长很重要，并且 atp 突变体是针对沙门氏菌感染的有效活减毒疫苗。
