炭疽杆菌转录阻遏蛋白 PagR 的晶体结构。
Crystal structure of the transcriptional repressor PagR of Bacillus anthracis.
机构信息
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, 4301 W Markham St, Little Rock, AR 72205-7199, USA.
出版信息
Microbiology (Reading). 2010 Feb;156(Pt 2):385-391. doi: 10.1099/mic.0.033548-0. Epub 2009 Nov 19.
Abstract
PagR is a transcriptional repressor in Bacillus anthracis that controls the chromosomal S-layer genes eag and sap, and downregulates the protective antigen pagA gene by direct binding to their promoter regions. The PagR protein sequence is similar to those of members of the ArsR repressor family involved in the repression of arsenate-resistance genes in numerous bacteria. The crystal structure of PagR was solved using multi-wavelength anomalous diffraction (MAD) techniques and was refined with 1.8 A resolution diffraction data. The PagR molecules form dimers, as observed in all SmtB/ArsR repressor family proteins. In the crystal lattice four PagR dimers pack together to form an inactive octamer. Model-building studies suggest that the dimer binds to a DNA duplex with a bend of around 4 degrees.
摘要
PagR 是炭疽芽胞杆菌中的一种转录阻遏物，它控制着染色体 S 层基因 eag 和 sap，并通过直接结合它们的启动子区域下调保护性抗原 pagA 基因。PagR 蛋白序列与参与许多细菌中砷酸盐抗性基因抑制的 ArsR 阻遏物家族成员的序列相似。使用多波长异常衍射 (MAD) 技术解决 PagR 的晶体结构，并使用 1.8A 分辨率衍射数据进行了细化。如在所有 SmtB/ArsR 阻遏物家族蛋白中观察到的那样，PagR 分子形成二聚体。在晶格中，四个 PagR 二聚体聚集在一起形成无活性的八聚体。模型构建研究表明，二聚体与具有约 4 度弯曲的 DNA 双链结合。
相似文献
1
Crystal structure of the transcriptional repressor PagR of Bacillus anthracis.炭疽杆菌转录阻遏蛋白 PagR 的晶体结构。
Microbiology (Reading). 2010 Feb;156(Pt 2):385-391. doi: 10.1099/mic.0.033548-0. Epub 2009 Nov 19.
2
Autogenous regulation of the Bacillus anthracis pag operon.炭疽芽孢杆菌pag操纵子的自体调节
J Bacteriol. 1999 Aug;181(15):4485-92. doi: 10.1128/JB.181.15.4485-4492.1999.
3
Overlapping and Distinct Functions of the Paralogous PagR Regulators of Bacillus anthracis.炭疽杆菌旁系同源 PagR 调控因子的重叠和独特功能。
J Bacteriol. 2022 Sep 20;204(9):e0020822. doi: 10.1128/jb.00208-22. Epub 2022 Aug 25.
4
In vivo Bacillus anthracis gene expression requires PagR as an intermediate effector of the AtxA signalling cascade.在体内，炭疽芽孢杆菌基因表达需要PagR作为AtxA信号级联反应的中间效应物。
Int J Med Microbiol. 2004 Apr;293(7-8):619-24. doi: 10.1078/1438-4221-00306.
5
Identification of CodY targets in Bacillus anthracis by genome-wide in vitro binding analysis.通过全基因组体外结合分析鉴定炭疽杆菌中的 CodY 靶标。
J Bacteriol. 2013 Mar;195(6):1204-13. doi: 10.1128/JB.02041-12. Epub 2013 Jan 4.
6
A plasmid-encoded regulator couples the synthesis of toxins and surface structures in Bacillus anthracis.一种质粒编码的调节因子将炭疽芽孢杆菌中毒素的合成与表面结构联系起来。
Mol Microbiol. 2003 Feb;47(4):917-27. doi: 10.1046/j.1365-2958.2003.03345.x.
7
Control of virulence gene expression in Bacillus anthracis.炭疽芽孢杆菌毒力基因表达的调控
J Appl Microbiol. 1999 Aug;87(2):279-81. doi: 10.1046/j.1365-2672.1999.00887.x.
8
Bacillus anthracis Virulence Regulator AtxA Binds Specifically to the Promoter Region.炭疽杆菌毒力调节因子 AtxA 特异性结合于启动子区域。
J Bacteriol. 2019 Nov 5;201(23). doi: 10.1128/JB.00569-19. Print 2019 Dec 1.
9
Structure and function of the arginine repressor-operator complex from Bacillus subtilis.枯草芽孢杆菌精氨酸阻遏物-操纵基因复合物的结构与功能
J Mol Biol. 2008 May 30;379(2):284-98. doi: 10.1016/j.jmb.2008.03.007. Epub 2008 Mar 12.
10
Modeling of the structure and interactions of the B. anthracis antitoxin, MoxX: deletion mutant studies highlight its modular structure and repressor function.炭疽杆菌解毒蛋白 MoxX 的结构与相互作用建模：缺失突变体研究突出了其模块化结构和抑制子功能。
J Comput Aided Mol Des. 2011 Mar;25(3):275-91. doi: 10.1007/s10822-011-9419-z. Epub 2011 Feb 19.
引用本文的文献
1
Increased intracellular persulfide levels attenuate HlyU-mediated hemolysin transcriptional activation in .细胞内过硫化物水平升高会减弱HlyU介导的溶血素转录激活作用。
bioRxiv. 2023 Mar 13:2023.03.13.532278. doi: 10.1101/2023.03.13.532278.
2
Impact of a Novel PagR-like Transcriptional Regulator on Cereulide Toxin Synthesis in Emetic .新型 PagR 样转录调控因子对致吐型大肠埃希氏菌呕吐毒素合成的影响
Int J Mol Sci. 2022 Sep 29;23(19):11479. doi: 10.3390/ijms231911479.
3
ArsR Family Regulator MSMEG_6762 Mediates the Programmed Cell Death by Regulating the Expression of HNH Nuclease in Mycobacteria.ArsR家族调控因子MSMEG_6762通过调节分枝杆菌中HNH核酸酶的表达介导程序性细胞死亡。
Microorganisms. 2022 Jul 29;10(8):1535. doi: 10.3390/microorganisms10081535.
4
Overlapping and Distinct Functions of the Paralogous PagR Regulators of Bacillus anthracis.炭疽杆菌旁系同源 PagR 调控因子的重叠和独特功能。
J Bacteriol. 2022 Sep 20;204(9):e0020822. doi: 10.1128/jb.00208-22. Epub 2022 Aug 25.
5
Metallochaperones and metalloregulation in bacteria.细菌中的金属伴侣蛋白与金属调节
Essays Biochem. 2017 May 9;61(2):177-200. doi: 10.1042/EBC20160076.
6
Transcription factor-based biosensors enlightened by the analyte.由分析物启发的基于转录因子的生物传感器。
Front Microbiol. 2015 Jul 1;6:648. doi: 10.3389/fmicb.2015.00648. eCollection 2015.
7
Dual effects of single-walled carbon nanotubes coupled with near-infrared radiation on Bacillus anthracis spores: inactivates spores and stimulates the germination of surviving spores.单壁碳纳米管与近红外辐射对炭疽芽孢杆菌芽孢的双重作用：既能使芽孢失活，又能刺激存活芽孢的萌发。
J Biol Eng. 2013 Aug 21;7:19. doi: 10.1186/1754-1611-7-19. eCollection 2013.
8
Bartonella quintana deploys host and vector temperature-specific transcriptomes.五日热巴尔通体利用宿主和媒介的温度特异性转录组。
PLoS One. 2013;8(3):e58773. doi: 10.1371/journal.pone.0058773. Epub 2013 Mar 12.
9
The redox-sensing transcriptional regulator RexT controls expression of thioredoxin A2 in the cyanobacterium Anabaena sp. strain PCC 7120.氧化还原感应转录调节剂 RexT 控制蓝藻鱼腥藻 PCC 7120 中硫氧还蛋白 A2 的表达。
J Biol Chem. 2012 Nov 23;287(48):40433-40. doi: 10.1074/jbc.M112.384206. Epub 2012 Oct 1.
10
Characterization of a novel ArsR-like regulator encoded by Rv2034 in Mycobacterium tuberculosis.结核分枝杆菌 Rv2034 编码的一种新型 ArsR 样调控因子的特性研究。
PLoS One. 2012;7(4):e36255. doi: 10.1371/journal.pone.0036255. Epub 2012 Apr 27.
本文引用的文献
1
Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
Intrinsic curvature associated with the coordinately regulated anthrax toxin gene promoters.与协同调控的炭疽毒素基因启动子相关的内在曲率。
Microbiology (Reading). 2008 Aug;154(Pt 8):2501-2512. doi: 10.1099/mic.0.2007/016162-0.
3
BslA, a pXO1-encoded adhesin of Bacillus anthracis.BslA，一种炭疽芽孢杆菌pXO1编码的黏附素。
Mol Microbiol. 2008 Apr;68(2):504-15. doi: 10.1111/j.1365-2958.2008.06169.x.
4
The integration of macromolecular diffraction data.大分子衍射数据的整合
Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):48-57. doi: 10.1107/S0907444905039107. Epub 2005 Dec 14.
5
Crystal structure of the Staphylococcus aureus pI258 CadC Cd(II)/Pb(II)/Zn(II)-responsive repressor.金黄色葡萄球菌pI258 CadC镉（II）/铅（II）/锌（II）响应阻遏物的晶体结构
J Bacteriol. 2005 Jun;187(12):4214-21. doi: 10.1128/JB.187.12.4214-4221.2005.
6
SOLVE and RESOLVE: automated structure solution and density modification.SOLVE与RESOLVE：自动化结构解析与密度修正
Methods Enzymol. 2003;374:22-37. doi: 10.1016/S0076-6879(03)74002-6.
7
A metal-ligand-mediated intersubunit allosteric switch in related SmtB/ArsR zinc sensor proteins.相关SmtB/ArsR锌传感器蛋白中金属-配体介导的亚基间变构开关
J Mol Biol. 2003 Oct 31;333(4):683-95. doi: 10.1016/j.jmb.2003.09.007.
8
The SmtB/ArsR family of metalloregulatory transcriptional repressors: Structural insights into prokaryotic metal resistance.金属调节转录阻遏物的SmtB/ArsR家族：对原核生物金属抗性的结构见解。
FEMS Microbiol Rev. 2003 Jun;27(2-3):131-43. doi: 10.1016/S0168-6445(03)00054-8.
9
A plasmid-encoded regulator couples the synthesis of toxins and surface structures in Bacillus anthracis.一种质粒编码的调节因子将炭疽芽孢杆菌中毒素的合成与表面结构联系起来。
Mol Microbiol. 2003 Feb;47(4):917-27. doi: 10.1046/j.1365-2958.2003.03345.x.
10
Dimerization allows DNA target site recognition by the NarL response regulator.二聚化使NarL应答调节因子能够识别DNA靶位点。
Nat Struct Biol. 2002 Oct;9(10):771-8. doi: 10.1038/nsb845.
Zotero 插件