Ansari Anees A, Sumana G, Khan R, Malhotra B D
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi 110012, India.
J Nanosci Nanotechnol. 2009 Aug;9(8):4679-85. doi: 10.1166/jnn.2009.1085.
Hydrogen peroxide (H2O2) sensor has been fabricated by immobilizing horse radish peroxidase (HRP) onto polyaniline(PANI)-cerium oxide (CeO2) nano-composite film onto indium-tin-oxide (ITO) coated glass substrate using electrochemical technique. The HRP/PANI-CeO2/ITO bioelectrode has been characterized using scanning electron microscopy (SEM), Fourier Transform Infrared spectroscopy, UV-visible spectroscopy and electrochemical analysis, respectively. This electrode shows improved sensitivity (159.6 nA/mM) and detection limit (50 mM). Further this H2O2 sensor shows high HRP affinity to the PANI-CeO2 matrix as indicated by low value (2.9) mM of Michaelis-Menten constant (K(m)). This bioelectrode exhibits maximum response current at pH 7.0 and retains 90% of its initial response current after about 8 weeks.
采用电化学技术，将辣根过氧化物酶（HRP）固定在聚苯胺（PANI）-氧化铈（CeO₂）纳米复合膜上，制备了过氧化氢（H₂O₂）传感器，该复合膜位于氧化铟锡（ITO）涂层玻璃基板上。分别使用扫描电子显微镜（SEM）、傅里叶变换红外光谱、紫外可见光谱和电化学分析对HRP/PANI-CeO₂/ITO生物电极进行了表征。该电极显示出提高的灵敏度（159.6 nA/mM）和检测限（50 mM）。此外，该H₂O₂传感器对PANI-CeO₂基质显示出高HRP亲和力，米氏常数（K(m)）的值较低（2.9 mM）表明了这一点。该生物电极在pH 7.0时表现出最大响应电流，并且在约8周后保留其初始响应电流的90%。
