Broulík P D, Vondrová J, Růzicka P, Sedlácek R, Zíma T
Third Medical Clinic, First Medical Faculty, Charles University, Prague, Czech Republic.
Physiol Res. 2010;59(4):599-604. Epub 2009 Nov 20.
Alcohol use has been identified as a risk factor for the development of osteoporosis. Eight male Wistar rats at two months of age were alcoho-fed (7.6 g 95 % ethanol/kg b.w. per day) to evaluate the effects of long-term administration (three months) of alcohol in drinking water. We have used a dose which is considered to be comparable to a dose of 1 liter of wine or 2.5 liters of 12(°) beer used in male adults daily. The bones were tested mechanically by a three-point bending test in a Mini Bionix (MTS) testing system. The bones from alcohol-fed rats were characterized by a reduction in bone density as well as in ash, calcium and phosphate content. In alcohol-fed rats the reduction in bone mineral density (10 %) was reflected by about 12 % reduction of mechanical strength of femur (158+/-5.5 vs. 178+/-3.2 N/mm(2)). Alcohol significantly altered femoral cortical thickness. In our experiment alcohol itself did not exert any antiandrogenic effect and it did not produce changes in the weight of seminal vesicles. Liver function test (GGT, ALP, AST) did not differ between alcohol-fed rats and control rats. Alcohol-induced bone loss is associated with increased bone resorption and decreased bone formation. These results document the efficacy of alcohol at the dose of 7.6 g 95 % ethanol/kg b.w. to cause bone loss and loss of bone mechanical strength in intact rats. The results of the present study may be interpreted as supporting the hypothesis of alcohol as a risk factor for osteoporosis.
饮酒已被确定为骨质疏松症发生的一个风险因素。选用8只2月龄雄性Wistar大鼠，给予酒精喂养（每天7.6克95%乙醇/千克体重），以评估长期（三个月）饮用含酒精的水的影响。我们使用的剂量被认为与成年男性每日饮用1升葡萄酒或2.5升12度啤酒的剂量相当。在Mini Bionix（MTS）测试系统中通过三点弯曲试验对骨骼进行力学测试。酒精喂养大鼠的骨骼表现为骨密度以及灰分、钙和磷含量降低。在酒精喂养的大鼠中，骨矿物质密度降低（10%）反映在股骨力学强度降低约12%（158±5.5对178±3.2牛/平方毫米）。酒精显著改变了股骨皮质厚度。在我们的实验中，酒精本身没有发挥任何抗雄激素作用，也没有使精囊重量发生变化。酒精喂养大鼠和对照大鼠之间的肝功能测试（γ-谷氨酰转移酶、碱性磷酸酶、天冬氨酸转氨酶）没有差异。酒精诱导的骨质流失与骨吸收增加和骨形成减少有关。这些结果证明了7.6克95%乙醇/千克体重剂量的酒精在完整大鼠中导致骨质流失和骨力学强度丧失的效力。本研究结果可解释为支持酒精作为骨质疏松症风险因素的假说。
