Department of Obstetrics and Gynaecology, University of Würzburg, Josef-Schneider-Str, 4, D-97080 Würzburg, Germany.
Reprod Biol Endocrinol. 2009 Nov 23;7:132. doi: 10.1186/1477-7827-7-132.
Changes in the balance of decidual leucocyte populations may lead to an unfavourable uterine microenvironment which may be associated with the development of preeclampsia (PE). In this study, we therefore investigated the leucocyte subpopulations in decidual tissues of 33 women with preeclampsia and 66 control patients.
Decidua was either obtained via curettage during cesarean section or dissected from the surface of the basal plate of the placenta after spontaneous delivery. We used FACS analysis to quantify decidual leukocytes (CD45), NK cells (CD56+/CD16+ and CD56++/CD16-), antigen presenting cells (HLA-DR, DC-Sign, CD14) and T/B cells (CD8, CD4, alpha-beta-T-cell receptor, gamma-delta-T-cell receptor, CD25, CD19).
The number of decidual cytotoxic CD8+T-lymphocytes (P < 0.02), alpha-beta -T-cell receptor positive T cells (P < 0.03) and of CD56+/CD16+ NK cells (P < 0.03) was lower in decidua from women with PE than in decidua from control patients.
The observed reduction of specific leucocyte subsets could create a microenvironment which is unfavourable for an appropriate placentation and could thereby be involved in the development of preeclamptic symptoms.
蜕膜白细胞群体的平衡变化可能导致不利于子宫的微环境，这可能与子痫前期 (PE) 的发展有关。在这项研究中，我们因此研究了 33 例子痫前期患者和 66 例对照患者的蜕膜组织中的白细胞亚群。
蜕膜通过剖宫产时刮宫或在自然分娩后从胎盘基底板表面解剖获得。我们使用流式细胞术分析来定量蜕膜白细胞（CD45）、NK 细胞（CD56+/CD16+和 CD56++/CD16-）、抗原呈递细胞（HLA-DR、DC-Sign、CD14）和 T/B 细胞（CD8、CD4、α-β-T 细胞受体、γ-δ-T 细胞受体、CD25、CD19）。
与对照组患者相比，PE 患者的蜕膜中细胞毒性 CD8+T 淋巴细胞（P < 0.02）、α-β-T 细胞受体阳性 T 细胞（P < 0.03）和 CD56+/CD16+NK 细胞（P < 0.03）数量减少。
观察到的特定白细胞亚群减少可能会产生不利于适当胎盘形成的微环境，从而参与子痫前期症状的发展。
