NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), Kazusakamatari 2-5-8, Kisarazu, Chiba, 292-0818, Japan.
Institute of Microbiology and Biotechnology (IMBT), Vietnam National University, Hanoi (VNUH), Japan.
Int J Syst Evol Microbiol. 2010 Oct;60(Pt 10):2336-2340. doi: 10.1099/ijs.0.018127-0. Epub 2009 Nov 20.
A novel actinomycete, designated strain VN05A0561(T), was isolated from plant litter collected at Ba Be National Park, Vietnam. The substrate mycelia and spore chains fragmented in a manner similar to nocardioform actinomycetes; the spores had smooth surfaces and were rod-shaped. Strain VN05A0561(T) had the following chemotaxonomic characteristics: meso-diaminopimelic acid in the cell-wall peptidoglycan, arabinose and galactose as characteristic sugars, MK-8(H₄) as the major isoprenoid quinone, phosphatidylcholine as the diagnostic phospholipid and iso-C₁₆:₀ as the major cellular fatty acid. Strain VN05A0561(T) shared low levels of 16S rRNA gene sequence similarity (< 97 %) with the type strains of recognized species of the genus Pseudonocardia and could be differentiated from its closest phylogenetic relatives based on phenotypic characteristics. These results suggested that strain VN05A0561(T) represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia babensis sp. nov. is proposed. The type strain is VN05A0561(T) (=VTCC-A-1757(T)=NBRC 105793(T)).
一株新的放线菌，命名为 VN05A0561(T) 菌株，从越南巴贝国家公园采集的植物凋落物中分离得到。基内菌丝体和孢子链呈类似诺卡氏放线菌的片段化方式；孢子表面光滑，呈杆状。VN05A0561(T) 菌株具有以下化学分类学特征：细胞壁肽聚糖中的 meso-二氨基庚二酸、阿拉伯糖和半乳糖作为特征糖、MK-8(H₄) 作为主要异戊烯醌、磷脂酰胆碱作为诊断磷脂和 iso-C₁₆:₀ 作为主要细胞脂肪酸。VN05A0561(T) 菌株与假诺卡氏菌属公认种的模式菌株的 16S rRNA 基因序列相似性<97%，根据表型特征可将其与最接近的系统发育亲缘种区分开来。这些结果表明，VN05A0561(T) 菌株代表假诺卡氏菌属的一个新种，建议将其命名为 Babensis 假诺卡氏菌 sp. nov.。模式菌株为 VN05A0561(T) (=VTCC-A-1757(T)=NBRC 105793(T))。
