Sonam Wangchen, Liu Yongqin, Ren Luming
State Key Laboratory of Tibetan Plateau Earth System, Environment and Resources (TPESER), Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Plants (Basel). 2025 Apr 11;14(8):1190. doi: 10.3390/plants14081190.
Plant microbiomes provide significant fitness advantages to their plant hosts, especially in the sub-nival belt. Studies to date have primarily focused on belowground communities in this region. Here, we utilized high-throughput DNA sequencing to quantify bacterial communities in the rhizosphere soil as well as in the root and leaf endosphere compartments of to uncover the differentiation and interconnections of these bacterial communities along the soil-to-plant continuum. Our findings reveal that the bacterial communities exhibit notable variation across different plant compartment niches: the rhizosphere soil, root endosphere, and leaf endosphere. There was a progressive decline in diversity, network complexity, network modularity, and niche breadth from the rhizosphere soil to the root endosphere, and further to the leaf endosphere. Conversely, both the host plant selection effect and the stability of these communities showed an increasing trend. Total nitrogen and total potassium emerged as crucial factors accounting for the observed differences in diversity and composition, respectively. Additionally, 3.6% of the total amplicon sequence variants (ASVs) were shared across the rhizosphere soil, root endosphere, and leaf endosphere. Source-tracking analysis further revealed bacterial community migration among these compartments. The genera , , , , and constituted the core of the bacterial microbiome. These taxa are shared across all three compartment niches and function as key connector species. Notably, stands out as the predominant taxon among these bacteria, with nitrogen being the most significant factor influencing its relative abundance. These findings deepen our understanding of the assembly principles and ecological dynamics of the plant microbiome in the sub-nival belt, offering an integrated framework for its study.
植物微生物群为其寄主植物提供了显著的适应性优势，尤其是在亚雪带。迄今为止的研究主要集中在该地区的地下群落。在这里，我们利用高通量DNA测序定量分析了根际土壤以及[植物名称]的根和叶内圈隔室中的细菌群落，以揭示这些细菌群落在从土壤到植物的连续体中的分化和相互联系。我们的研究结果表明，细菌群落在不同的植物隔室生态位（根际土壤、根内圈和叶内圈）中表现出显著差异。从根际土壤到根内圈，再到叶内圈，多样性、网络复杂性、网络模块性和生态位宽度逐渐下降。相反，寄主植物选择效应和这些群落的稳定性均呈上升趋势。总氮和总钾分别成为导致观察到的多样性和组成差异的关键因素。此外，在根际土壤、根内圈和叶内圈中，共有3.6%的扩增子序列变体（ASV）。溯源分析进一步揭示了这些隔室之间的细菌群落迁移。[细菌属名称1]、[细菌属名称2]、[细菌属名称3]、[细菌属名称4]和[细菌属名称5]构成了细菌微生物群的核心。这些分类群在所有三个隔室生态位中都有共享，并作为关键的连接物种发挥作用。值得注意的是，[细菌属名称1]在这些细菌中是最主要的分类群，氮是影响其相对丰度的最重要因素。这些发现加深了我们对亚雪带植物微生物群组装原理和生态动态的理解，为其研究提供了一个综合框架。
