Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad - 500 007, India.
Int J Syst Evol Microbiol. 2010 Oct;60(Pt 10):2320-2325. doi: 10.1099/ijs.0.020230-0. Epub 2009 Nov 20.
A novel Gram-negative, rod-shaped, non-motile bacterium, designated strain LW7(T), was isolated from a water sample collected at a depth of 4.5 m from Lonar Lake in Buldhana district, Maharastra, India. The cell suspension was dark-reddish orange due to the presence of carotenoids. The fatty acids were dominated by large amounts of iso-C₁₅:₀ (59.6 %) and iso-C₁₇:₀ 3-OH (8.9 %). Strain LW7(T) contained MK-4 and MK-5 as the major respiratory quinones and phosphatidylglycerol and phosphatidylethanolamine as the major phospholipids. 16S rRNA gene sequence analysis indicated that Belliella baltica, a member of family 'Cyclobacteriaceae' (phylum Bacteroidetes), is the closest related species, with a sequence similarity of 94.0 % to the type strain. Other members of the family 'Cyclobacteriaceae' had sequence similarities of < 93.3 %. Based on the above-mentioned phenotypic and phylogenetic characteristics, strain LW7(T) is proposed as a representative of a new genus and species, Nitritalea halalkaliphila gen. nov., sp. nov. The type strain of Nitritalea halalkaliphila is LW7(T) (=CCUG 57665(T) =JCM 15946(T) =NCCB 100279(T)). The genomic DNA G+C of strain LW7(T) is 49 mol%.
一种新型革兰氏阴性、杆状、非运动性细菌，命名为 LW7(T) 菌株，从印度马哈拉施特拉邦布尔丹那区 Lonar 湖 4.5 米深处的水样中分离得到。由于类胡萝卜素的存在，细胞悬浮液呈暗橙红色。脂肪酸主要由大量的异 C₁₅:₀（59.6 %）和异 C₁₇:₀ 3-OH（8.9 %）组成。LW7(T) 菌株含有 MK-4 和 MK-5 作为主要的呼吸醌，磷脂酰甘油和磷脂酰乙醇胺作为主要的磷脂。16S rRNA 基因序列分析表明，贝氏菌属（家族 'Cyclobacteriaceae'）的成员 Belliella baltica 是最接近的相关物种，与模式菌株的序列相似度为 94.0 %。家族 'Cyclobacteriaceae' 的其他成员的序列相似度<93.3 %。基于上述表型和系统发育特征，LW7(T) 菌株被提议为一个新属和新种的代表，Nitritalea halalkaliphila gen. nov.，sp. nov.。Nitritalea halalkaliphila 的模式菌株是 LW7(T)（=CCUG 57665(T)=JCM 15946(T)=NCCB 100279(T)）。LW7(T) 菌株的基因组 DNA G+C 含量为 49 mol%。
