MTCC-Microbial Type Culture Collection & Gene Bank, CSIR-Institute of Microbial Technology, Chandigarh, 160036, India.
Antonie Van Leeuwenhoek. 2013 Mar;103(3):519-25. doi: 10.1007/s10482-012-9834-8. Epub 2012 Nov 5.
A Gram-negative, rod shaped, motile bacterium, was isolated from a marine solar saltern sample collected from Kakinada, India. Strain AK2(T) was determined to be positive for nitrate reduction, catalase, Ala-Phe-Pro-arylamidase, β-galactosidase, β-N-acetylglucosaminidase, β-glucosidase, β-xylosidase, α-glucosidase, α-galactosidase and phosphatase activities, hydrolysis of aesculin, Tween 20/40/60/80 and urea. It was determined to be negative for oxidase, lysine decarboxylase and ornithine decarboxylase activities and could not hydrolyze agar, casein, gelatin and starch. The predominant fatty acids were identified as iso-C(15:0) (28.2 %), anteiso-C(15:0) (23.2 %), iso-C(13:0) (19.9 %) and iso-C(15:0) 3-OH (13.9 %). Strain AK2(T) was found to contain menaquinone with seven isoprene units (MK-7) as the sole respiratory quinone and phosphatidylethanolamine, one unidentified phospholipid and three unidentified lipids as polar lipids. The 16S rRNA gene sequence analysis indicated the strain AK2(T) as a member of the genus Marinilabilia and is closely related to Marinilabilia salmonicolor with pair-wise sequence similarity of 98.2 %. Phylogenetic analysis of 16S rRNA gene revealed that the strain AK2(T) clustered with M. salmonicolor. However, DNA-DNA hybridization with M. salmonicolor JCM 21150(T) showed a relatedness of 48 ± 0.5 % with respect to strain AK2(T). The DNA G+C content of the strain was determined to be 40.2 mol%. Based on the phenotypic characteristics and phylogenetic inference, it is proposed that the strain AK2(T) represents a novel species of the genus Marinilabilia, for which the name Marinilabilia nitratireducens sp. nov. is proposed. The type strain of M. nitratireducens sp. nov. is AK2(T) (= MTCC 11402(T) = JCM 17679(T)).
从印度卡基纳达的海洋盐沼样本中分离到一株革兰氏阴性、杆状、能动的细菌。菌株 AK2(T) 被确定为硝酸盐还原、过氧化氢酶、Ala-Phe-Pro-芳基酰胺酶、β-半乳糖苷酶、β-N-乙酰葡萄糖胺酶、β-葡萄糖苷酶、β-木糖苷酶、α-葡萄糖苷酶、α-半乳糖苷酶和磷酸酶活性、马栗树皮苷、吐温 20/40/60/80 和尿素水解阳性，氧化酶、赖氨酸脱羧酶和鸟氨酸脱羧酶活性阴性，不能水解琼脂、酪蛋白、明胶和淀粉。优势脂肪酸鉴定为 iso-C(15:0) (28.2 %)、anteiso-C(15:0) (23.2 %)、iso-C(13:0) (19.9 %)和 iso-C(15:0) 3-OH (13.9 %)。菌株 AK2(T) 被发现含有七异戊二烯单位的menaquinone (MK-7) 作为唯一的呼吸醌，以及磷脂酰乙醇胺、一种未鉴定的磷脂和三种未鉴定的脂质作为极性脂质。16S rRNA 基因序列分析表明，菌株 AK2(T) 是 Marinilabilia 属的一个成员，与 Marinilabilia salmonicolor 的序列相似性为 98.2 %。16S rRNA 基因的系统发育分析表明，菌株 AK2(T) 与 M. salmonicolor 聚为一簇。然而，与 M. salmonicolor JCM 21150(T) 的 DNA-DNA 杂交显示，菌株 AK2(T) 与 M. salmonicolor 的相关性为 48 ± 0.5 %。菌株的 DNA G+C 含量为 40.2 mol%。基于表型特征和系统发育推断，建议菌株 AK2(T) 代表 Marinilabilia 属的一个新种，命名为 Marinilabilia nitratireducens sp. nov.。M. nitratireducens sp. nov. 的模式菌株为 AK2(T)=MTCC 11402(T)=JCM 17679(T)。
