Centre de Recherche en Automatique de Nancy-Nancy-University-CNRS-Centre Alexis Vautrin, avenue de Bourgogne, 54511 Vandoeuvre-lès-Nancy Cedex, France.
Mol Imaging Biol. 2010 Aug;12(4):394-405. doi: 10.1007/s11307-009-0288-y. Epub 2009 Nov 21.
This study compares fluorescence imaging to mass spectroscopy (inductively coupled plasma-mass spectroscopy, ICP-MS) for detection of quantum dots (QDs) in sentinel lymph node (LN) mapping of breast cancer.
We study the accumulation of near-infrared-emitting QDs into regional LNs and their whole-body biodistribution in mice after subcutaneous injection, using in vivo fluorescence imaging and ex vivo elemental analysis by ICP-MS.
We show that the QD accumulation in regional LNs is detectable by fluorescence imaging as early as 5 min post-delivery. Their concentration reaches a maximum at 4 h then decreases over a 10-day observation period. These data are confirmed by ICP-MS. The QD uptake in other organs, assessed by ICP-MS, increases steadily over time; however, its overall level remains rather low.
Fluorescence imaging can be used as a non-invasive alternative to ICP-MS to follow the QD accumulation kinetics into regional LNs.
本研究比较了荧光成像与质谱（电感耦合等离子体质谱法，ICP-MS）在乳腺癌前哨淋巴结（SLN）示踪中检测量子点（QD）的效果。
我们通过皮下注射，在体内荧光成像和 ICP-MS 体外元素分析中研究了近红外发射 QD 在后注射后在局部淋巴结中的积累及其全身生物分布。
我们发现，在注射后 5 分钟即可通过荧光成像检测到 QD 在局部淋巴结中的积累。它们的浓度在 4 小时达到最大值，然后在 10 天的观察期内逐渐下降。这些数据通过 ICP-MS 得到了证实。通过 ICP-MS 评估，其他器官中的 QD 摄取量随时间稳定增加；然而，其整体水平仍然相当低。
荧光成像可以作为 ICP-MS 的非侵入性替代方法，用于跟踪 QD 向局部淋巴结中的积累动力学。
