福格特（Fuchs）异色性虹膜睫状体炎患者眼内针对风疹病毒和其他微生物的抗体合成。
Intraocular antibody synthesis against rubella virus and other microorganisms in Fuchs' heterochromic cyclitis.
机构信息
Augenklinik Charité-Universitätsmedizin Berlin (CVK) Germany, Augustenburger Platz 1, 13353 Berlin, Germany.
出版信息
Graefes Arch Clin Exp Ophthalmol. 2010 Apr;248(4):565-71. doi: 10.1007/s00417-009-1239-7.
INTRODUCTION
Fuchs' heterochromic cyclitis (FHC) is a common intraocular disease of uncertain etiology that has recently been related to rubella virus (RV) infection.
METHODS
We investigated the synthesis of RV-specific IgG using aqueous humor and serum samples from 63 consecutive patients with FHC. In addition, intraocular immunoglobulin G production against herpes simplex virus (HSV), varicella zoster virus (VZV), cytomegalovirus (CMV) and toxoplasma gondii was determined. In 20 patients, the detection of RV RNA was additionally performed by RT-PCR on the E1 gene. Forty-six patients with HSV- and VZV-associated uveitis, HLA B-27 positive anterior uveitis, and Posner-Schlossman syndrome served as controls.
RESULTS
Specific intraocular antibody synthesis against RV was confirmed in all 63 FHC patients, whereas none of the 46 controls was positive for RV IgG. Interestingly, in 11 patients with positive RV IgG synthesis, additional HSV (eight), VZV (one) and CMV (two) specific antibodies could be detected. Only twice was viral RNA detectable by PCR in a patient with FHC.
CONCLUSIONS
In this largest reported series of FHC patients, we detected a strong association between FHC and intraocular antibody synthesis against rubella virus. Furthermore, in 11 patients, it was possible to confirm an additional intraocular antibody synthesis, in particular HSV. PCR-positive results in the aqueous humor were exclusively obtained for RV. In contrast to other studies, the RV genome could only be identified in two patients (10%).
简介
Fuchs 异色性睫状体炎（FHC）是一种常见的病因不明的眼内疾病，最近与风疹病毒（RV）感染有关。
方法
我们研究了 63 例连续 FHC 患者的房水和血清样本中 RV 特异性 IgG 的合成。此外，还测定了针对单纯疱疹病毒（HSV）、水痘带状疱疹病毒（VZV）、巨细胞病毒（CMV）和弓形虫的眼内免疫球蛋白 G 产生情况。在 20 例患者中，还通过 RT-PCR 检测了 E1 基因上的 RV RNA。46 例 HSV 和 VZV 相关性葡萄膜炎、HLA B-27 阳性前葡萄膜炎和 Posner-Schlossman 综合征患者作为对照。
结果
所有 63 例 FHC 患者均证实存在针对 RV 的特异性眼内抗体合成，而 46 例对照中无一例 RV IgG 阳性。有趣的是，在 11 例 RV IgG 合成阳性的患者中，还可以检测到其他 HSV（8 例）、VZV（1 例）和 CMV（2 例）特异性抗体。只有 1 例 FHC 患者的 PCR 可检测到病毒 RNA。
结论
在本报告的最大系列 FHC 患者中，我们发现 FHC 与针对风疹病毒的眼内抗体合成之间存在强烈关联。此外，在 11 例患者中，还可以证实存在其他眼内抗体合成，特别是 HSV。仅在 2 例患者（10%）的房水中获得了 PCR 阳性结果。与其他研究不同，仅在 2 例患者（10%）中鉴定出了 RV 基因组。
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