在青光眼的活体小鼠模型中快速、无创地对视网膜神经节细胞进行成像。
Rapid and noninvasive imaging of retinal ganglion cells in live mouse models of glaucoma.
机构信息
Bernard and Shirlee Brown Glaucoma Laboratory, Departments of Ophthalmology, Pathology and Cell Biology, Columbia University, 630 West 168th Street, Box 18, New York, NY 10032, USA.
出版信息
Mol Imaging Biol. 2010 Aug;12(4):386-93. doi: 10.1007/s11307-009-0292-2. Epub 2009 Nov 24.
PURPOSE
We report a noninvasive method for the monitoring of retinal ganglion cell (RGC) survival in live mice utilizing standard fluorescence microscopy.
PROCEDURES
Transgenic mice expressing cyan fluorescent protein (CFP) under the regulation of an RGC-specific promoter Thy1 were used in this study.
RESULTS
We established that Thy1-CFP expression is a quantitative reflection of the number of surviving RGCs, the fluorescence emission is stable for at least a year and that the loss of fluorescence correlates directly to glaucomatous damage. In high pressure glaucoma model, the peripheral retina is preferentially affected.
CONCLUSIONS
Our live-imaging technique allows for the longitudinal assessment of RGC survival from the same animal. Noninvasive monitoring of neuronal cell death and survival is a powerful technique that would allow investigators to validate new potential glaucoma therapy based on neuroprotection.
目的
我们报告了一种利用标准荧光显微镜监测活体小鼠视网膜神经节细胞（RGC）存活的非侵入性方法。
方法
本研究使用了在 RGC 特异性启动子 Thy1 调控下表达青色荧光蛋白（CFP）的转基因小鼠。
结果
我们确定 Thy1-CFP 表达是存活 RGC 数量的定量反映，荧光发射至少稳定一年，并且荧光损失与青光眼损伤直接相关。在高压青光眼模型中，周边视网膜优先受到影响。
结论
我们的活体成像技术允许从同一动物进行 RGC 存活的纵向评估。对神经元细胞死亡和存活的非侵入性监测是一种强大的技术，它将允许研究人员根据神经保护来验证新的潜在青光眼治疗方法。
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