Boudreau M, Higgins R, Mittal K R
Faculté de Médecine Vétérinaire, Université de Montréal, St. Hyacinthe, Québec, Canada.
J Clin Microbiol. 1991 Jan;29(1):54-8. doi: 10.1128/jcm.29.1.54-58.1991.
Sixty-two isolates of Campylobacter cryaerophila were recovered from aborted porcine and bovine fetuses, from porcine, bovine, and equine feces, and from different tissues of a dead piglet. Phenotypic characterization was carried out on all isolates, and the results were compared with those obtained with the reference strains of C. cryaerophila, C. jejuni, C. coli, C. laridis, and C. hyointestinalis. The ability of C. cryaerophila strains to grow under aerobic conditions at 16 degrees C was found to be most useful in differentiating them from strains of other Campylobacter species. Studies were undertaken to develop a serotyping system for C. cryaerophila on the basis of the Lior serotyping system for C. jejuni and C. coli by use of a tube agglutination test with formalinized whole-cell (FWC) and boiled whole-cell (BWC) antigens. Antisera against 18 strains of C. cryaerophila were produced in rabbits. Thirty-five percent of C. cryaerophila strains were typed with the FWC suspension as an antigen, and 61% were typed with the BWC suspension as an antigen. None of the C. cryaerophila strains tested autoagglutinated in saline. BWC antigens of C. jejuni, C. coli, and C. laridis cross-reacted with C. cryaerophila, whereas FWC antigens did not cross-react. Neither FWC nor BWC antigens of C. hyointestinalis reacted with C. cryaerophila antisera.
从流产的猪和牛胎儿、猪、牛和马的粪便以及一头死仔猪的不同组织中分离出62株嗜低温弯曲杆菌。对所有分离株进行了表型特征分析，并将结果与嗜低温弯曲杆菌、空肠弯曲杆菌、大肠弯曲杆菌、拉氏弯曲杆菌和猪肠弯曲杆菌参考菌株的结果进行了比较。发现嗜低温弯曲杆菌菌株在16℃有氧条件下生长的能力最有助于将它们与其他弯曲杆菌属菌株区分开来。基于空肠弯曲杆菌和大肠弯曲杆菌的Lior血清分型系统，通过使用甲醛化全细胞（FWC）和煮沸全细胞（BWC）抗原的试管凝集试验，开展了嗜低温弯曲杆菌血清分型系统的研究。用兔制备了针对18株嗜低温弯曲杆菌的抗血清。35%的嗜低温弯曲杆菌菌株以FWC悬液作为抗原进行分型，61%以BWC悬液作为抗原进行分型。所检测的嗜低温弯曲杆菌菌株在盐水中均不发生自身凝集。空肠弯曲杆菌、大肠弯曲杆菌和拉氏弯曲杆菌的BWC抗原与嗜低温弯曲杆菌发生交叉反应，而FWC抗原不发生交叉反应。猪肠弯曲杆菌的FWC和BWC抗原均不与嗜低温弯曲杆菌抗血清发生反应。
