Senoglu Mehmet, Nacitarhan Vedat, Kurutas Ergul Belge, Senoglu Nimet, Altun Idris, Atli Yalcin, Ozbag Davut
Department of Neurosurgery, Kahramanmaras Sutcu Imam University Faculty of Medicine, Kahramanmaras, Turkey.
J Brachial Plex Peripher Nerve Inj. 2009 Nov 25;4:22. doi: 10.1186/1749-7221-4-22.
Crush injury to the sciatic nerve causes oxidative stress. Alfa Lipoic acid (a-LA) is a neuroprotective metabolic antioxidant. This study was designed to investigate the antioxidant effects of pretreatment with a-LA on the crush injury of rat sciatic nerve.
Forty rats were randomized into four groups. Group I and Group II received saline (2 ml, intraperitoneally) and a-LA (100 mg/kg, 2 ml, intraperitoneally) in the groups III and IV at the 24 and 1 hour prior to the crush injury. In groups II, III and IV, the left sciatic nerve was exposed and compressed for 60 seconds with a jeweler's forceps. In Group I (n = 10), the sciatic nerve was explored but not crushed. In all groups of rats, superoxide dismutase (SOD) and catalase (CAT) activities, as well as malondialdehyde (MDA) levels were measured in samples of sciatic nerve tissue.
Compared to Group I, Group II had significantly decreased tissue SOD and CAT activities and elevated MDA levels indicating crush injury (p < 0.05). In the a-LA treatment groups (groups III and IV), tissue CAT and SOD activities were significantly increased and MDA levels significantly decreased at the first hour (p < 0.05) and on the 3rd day (p < 0.05). There was no significant difference between a-LA treatment groups (p > 0.05).
A-LA administered before crush injury of the sciatic nerve showed significant protective effects against crush injury by decreasing the oxidative stress. A-LA should be considered in the treatment of peripheral nerve injuries, but further studies are needed to explain the mechanism of its neuroprotective effects.
坐骨神经挤压伤会导致氧化应激。α-硫辛酸（α-LA）是一种具有神经保护作用的代谢性抗氧化剂。本研究旨在探讨α-LA预处理对大鼠坐骨神经挤压伤的抗氧化作用。
40只大鼠随机分为四组。在挤压伤前24小时和1小时，第一组和第二组腹腔注射生理盐水（2 ml），第三组和第四组腹腔注射α-LA（100 mg/kg，2 ml）。在第二组、第三组和第四组中，暴露左侧坐骨神经并用珠宝商用镊子挤压60秒。第一组（n = 10），仅探查坐骨神经但不进行挤压。在所有大鼠组中，测量坐骨神经组织样本中的超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性以及丙二醛（MDA）水平。
与第一组相比，第二组的组织SOD和CAT活性显著降低，MDA水平升高，表明存在挤压伤（p < 0.05）。在α-LA治疗组（第三组和第四组）中，在第1小时（p < 0.05）和第3天（p < 0.05），组织CAT和SOD活性显著增加，MDA水平显著降低。α-LA治疗组之间无显著差异（p > 0.05）。
在坐骨神经挤压伤前给予α-LA通过降低氧化应激对挤压伤显示出显著的保护作用。在周围神经损伤的治疗中应考虑使用α-LA，但需要进一步研究来解释其神经保护作用的机制。
