Department of Chemistry, Liaoning University, Shenyang, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2010 Jan;75(1):366-74. doi: 10.1016/j.saa.2009.10.042. Epub 2009 Nov 4.
Sonodynamic therapy (SDT) is an attractive antitumor treatment for recent years. In this paper, sodium magnesium chlorophyllin (SMC) as a sonosensitizer combining with ultrasonic (US) irradiation to damage bovine serum albumin (BSA) has been investigated by fluorescence and UV-vis spectroscopy. The interaction of BSA with SMC was studied by the quenching of intrinsic fluorescence at varying temperature. The quenching constants (K(SV)), effective binding constants (K(A)), apparent association constants (K(a)) and binding site numbers were determined. The results indicated the quenching mechanism is a static procedure. Thermodynamic parameters show that the interactions involve hydrogen bonds, hydrophobic interactions, electrostatic interactions and complexations. The binding distance is 3.533 nm. The synergistic effect of SMC and ultrasound was estimated including the study of damage conditions. Synchronous fluorescence spectra indicate the damage to Trp residues is more serious. This paper may offer some valuable references for using spectroscopy method to study the application of chlorophyll derivatives in antitumor treatment.
声动力学疗法（SDT）是近年来一种有吸引力的抗肿瘤治疗方法。本文以叶绿素镁钠盐（SMC）作为声敏剂，结合超声（US）辐照，用荧光和紫外-可见光谱法研究了其对牛血清白蛋白（BSA）的损伤作用。通过在不同温度下测定内源荧光的猝灭，研究了 SMC 与 BSA 的相互作用。测定了猝灭常数（K（SV））、有效结合常数（K（A））、表观结合常数（K（a））和结合位点数。结果表明，猝灭机制是静态过程。热力学参数表明，相互作用涉及氢键、疏水相互作用、静电相互作用和络合。结合距离为 3.533nm。通过研究损伤条件，评估了 SMC 和超声的协同效应。同步荧光光谱表明色氨酸残基的损伤更为严重。本文可为利用光谱法研究叶绿素衍生物在抗肿瘤治疗中的应用提供一些有价值的参考。
