College of Chemistry, Liaoning University, Shenyang 110036, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2011 Sep;79(5):849-57. doi: 10.1016/j.saa.2011.05.085. Epub 2011 Jun 1.
In this paper, the chlorophyll derivatives, metallochlorophyllin (Chl-M) (M=Fe, Zn and Cu) including chlorophyllin iron (Chl-Fe), chlorophyllin zinc (Chl-Zn) and chlorophyllin copper (Chl-Cu), were adopted as sonosensitizers to combine with ultrasonic irradiation, and the sonodynamic damage of bovine serum albumin (BSA) was investigated. At first, the interaction of Chl-M with BSA was studied by fluorescence spectroscopy. The results show that the quenching mechanism belongs to a static process and among them the affinity of Chl-Fe to BSA is the most obvious. Then, some influence factors on the sonodynamic damage of BSA molecules in the presence of Chl-M under ultrasonic irradiation were also studied. Synchronous fluorescence spectra show that the binding and damage sites of Chl-M to BSA molecule are mainly on the tryptophan (Trp) residues. The generation of ROS in Chl-M sonodynamic process is estimated by the method of Oxidation-Extraction Spectrometry (OEP). This paper may offer some valuable references for the study of the sonodynamic activity of Chl-M and the effect of the central metals. Synchronously, it contributes to the application of Chl-M in SDT for tumor treatment.
本文采用叶绿素衍生物，金属叶绿素（Chl-M）（M=Fe、Zn 和 Cu），包括叶绿素铁（Chl-Fe）、叶绿素锌（Chl-Zn）和叶绿素铜（Chl-Cu）作为声敏剂与超声辐射相结合，研究了牛血清白蛋白（BSA）的声动力学损伤。首先，通过荧光光谱研究了 Chl-M 与 BSA 的相互作用。结果表明，猝灭机制属于静态过程，其中 Chl-Fe 与 BSA 的亲和力最为明显。然后，还研究了超声辐射下 Chl-M 存在时 BSA 分子声动力学损伤的一些影响因素。同步荧光光谱表明，Chl-M 与 BSA 分子的结合和损伤部位主要在色氨酸（Trp）残基上。通过氧化提取光谱法（OEP）估算了 Chl-M 声动力学过程中 ROS 的产生。本文可为 Chl-M 的声动力学活性及中心金属影响的研究提供一些有价值的参考。同时，为 Chl-M 在肿瘤治疗的 SDT 中的应用提供了依据。
