School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore.
Biomaterials. 2010 Mar;31(7):1533-45. doi: 10.1016/j.biomaterials.2009.11.022. Epub 2009 Nov 28.
In this study, the effect of surface topography on fibrinogen and platelet adsorption was investigated. High aspect ratio surface features, in the submicron to nanometer range, were constructed on the poly- (lactic-co-glycolic-acid) (PLGA) films. The topographic surfaces were fabricated by solvent-mediated polymer casting on a master template. Fibrinogen adsorption and platelets adhesion on these topographic surfaces were quantified by enzyme linked immunosorbent assay (ELISA) and lactate dehydrogenase (LDH) assay respectively, while the activation of platelets was quantified by flow cytometric analysis using fluorescein isothiocyanate (FITC) tagging. The lowest fibrinogen adsorption amount and platelet activity was observed on surfaces with specific topographical features in the submicron range with a significant reduction in adhesion when compared to the pristine PLGA films. The topographical parameters found to induce low levels of fibrinogen adsorption and platelet response were high aspect ratio structures (>3:1) with reduced interspacing (<200 nm) or high density. The results signify that topographical manipulation of thrombogenic surfaces of biodegradable polymers is a feasible approach for reducing their thrombogenicity.
在这项研究中，研究了表面形貌对纤维蛋白原和血小板吸附的影响。在聚（乳酸-共-乙醇酸）（PLGA）薄膜上构建了亚微米到纳米范围内高纵横比的表面形貌特征。通过在主模板上的溶剂介导的聚合物铸造来制造形貌表面。通过酶联免疫吸附测定（ELISA）和乳酸脱氢酶（LDH）测定分别定量了这些形貌表面上的纤维蛋白原吸附和血小板黏附，而通过使用异硫氰酸荧光素（FITC）标记的流式细胞术分析定量了血小板的激活。在具有特定亚微米范围内形貌特征的表面上观察到最低的纤维蛋白原吸附量和血小板活性，与原始 PLGA 薄膜相比，黏附显著减少。发现可诱导低纤维蛋白原吸附和血小板反应的形貌参数是具有高纵横比结构（>3:1）和减小的间隔（<200nm）或高密度。结果表明，生物可降解聚合物的血栓形成表面的形貌处理是降低其血栓形成性的可行方法。
