Southwest Foundation for Biomedical Research, San Antonio, Texas, USA.
Am J Hum Biol. 2010 Jul-Aug;22(4):444-8. doi: 10.1002/ajhb.21015.
The aim of this study was to analyze the heritability and the presence of pleiotropic effects on subfractions of high-density lipoproteins (HDLs) as measured by nuclear magnetic resonance (NMR), parameters for adiposity, and glucose metabolism in adult Alaskan Eskimos. The present family study included 1,214 adult Alaskan Eskimos (537 male/677 female). Body weight, height, circumferences, selected skinfolds, and blood pressure were measured in all participants. Blood samples were collected under fasting conditions for the isolation of plasma. Glucose, insulin, subclasses and size of lipoproteins, triglycerides, total, and HDL cholesterol and lipoprotein (a) were measured in plasma. HbA1c was measured in total blood. Univariate and bivariate quantitative genetic analyses were conducted between HDL subclasses and size and the anthropometric and biochemical measures using the variance decomposition approach. Variation in all the analyzed traits exhibits a significant genetic component. Heritabilities ranged between 0.18 +/- 0.11 for LDL(2) (intermediate) and 0.89 +/- 0.07 for small HDL. No common genetic effects were found on the HDL subclasses (small, intermediate, and large). Small HDL particles were genetically correlated with LDL particles and HbA1c. Negative genetic correlations were observed between intermediate and large HDL subfractions, HDL size and measures of adiposity, and LDL and parameters for glucose metabolism (HbA1, insulin). These observations confirm the presence of possible pleiotropic effects on HDL, adiposity, and cardiovascular risk factors and provide novel insight on the relationship between HDL subclasses, adiposity, and glucose regulation.
本研究旨在分析通过核磁共振（NMR）测量的高密度脂蛋白（HDL）亚组分、肥胖参数和葡萄糖代谢在成年阿拉斯加爱斯基摩人中的遗传力和存在的多效性影响。本家族研究包括 1214 名成年阿拉斯加爱斯基摩人（537 名男性/677 名女性）。所有参与者均测量体重、身高、周长、选定的皮褶厚度和血压。在所有参与者空腹条件下采集血样以分离血浆。测量血浆中的葡萄糖、胰岛素、脂蛋白亚类和大小、甘油三酯、总胆固醇和 HDL 胆固醇和脂蛋白（a）。在全血中测量 HbA1c。使用方差分解方法在单变量和双变量定量遗传分析之间进行 HDL 亚组分和大小与人体测量和生化指标之间的分析。所有分析性状的变异性均表现出显著的遗传成分。遗传力范围在 LDL(2)（中间）的 0.18 +/- 0.11 和小 HDL 的 0.89 +/- 0.07 之间。在 HDL 亚组分（小、中、大）上未发现共同的遗传效应。小 HDL 颗粒与 LDL 颗粒和 HbA1c 具有遗传相关性。中间和大 HDL 亚组分、HDL 大小和肥胖参数以及 LDL 和葡萄糖代谢参数（HbA1、胰岛素）之间观察到负遗传相关性。这些观察结果证实了 HDL、肥胖和心血管危险因素的可能多效性影响，并提供了关于 HDL 亚组分、肥胖和葡萄糖调节之间关系的新见解。
