Etchebehere C, Pavan M E, Zorzópulos J, Soubes M, Muxí L
Departamento de Microbiología, Facultad de Química y Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
Int J Syst Bacteriol. 1998 Oct;48 Pt 4:1297-304. doi: 10.1099/00207713-48-4-1297.
A new anaerobic, proteolytic, moderately thermophilic bacterium, strain 3RT, was isolated from a methanogenic mesophilic reactor treating protein-rich wastewater. The cells were Gram-negative, non-spore-forming, non-motile rods. The DNA base composition was 43 mol% G + C. The optimum pH and temperature for growth were 7.0 and 55 degrees C respectively. The bacterium fermented gelatin, casein, bovine albumin, peptone and yeast extract. Glucose, fructose, sucrose, maltose and starch were poorly fermented. The major fermentation products from glucose were acetate, CO2 and H2 and, from gelatin, propionate was also detected. Growth on glucose was stimulated by thiosulfate, which was reduced to sulfide. Sulfate and nitrate were not reduced. 16S rRNA gene analysis revealed that the isolated bacterial strain was phylogenetically related to Coprothermobacter proteolyticus (96.3% sequence similarity), the only known species within the genus. DNA-DNA hybridization analysis demonstrated a very low level of homology, indicating that the isolated strain and C. proteolyticus were not related at species level. Therefore, it is proposed to classify the described strain in the genus Coprothermobacter as a new species, Coprothermobacter platensis. The type strain of C. platensis is strain 3RT (= DSM 11748T).
从处理富含蛋白质废水的产甲烷嗜温反应器中分离出一种新的厌氧、蛋白水解、中度嗜热细菌，菌株3RT。细胞为革兰氏阴性、不产芽孢、无运动性的杆菌。DNA碱基组成为43 mol% G + C。生长的最适pH和温度分别为7.0和55℃。该细菌能发酵明胶、酪蛋白、牛血清白蛋白、蛋白胨和酵母提取物。葡萄糖、果糖、蔗糖、麦芽糖和淀粉发酵能力较差。葡萄糖发酵的主要产物是乙酸盐、CO2和H2，明胶发酵还检测到丙酸盐。硫代硫酸盐刺激葡萄糖生长，硫代硫酸盐被还原为硫化物。不还原硫酸盐和硝酸盐。16S rRNA基因分析表明，分离出的菌株在系统发育上与解蛋白粪嗜热杆菌（序列相似性96.3%）相关，是该属中唯一已知的物种。DNA-DNA杂交分析显示同源性水平非常低，表明分离出的菌株和解蛋白粪嗜热杆菌在种水平上不相关。因此，建议将所描述的菌株归为粪嗜热杆菌属的一个新物种，即普拉滕斯粪嗜热杆菌。普拉滕斯粪嗜热杆菌的模式菌株是菌株3RT（= DSM 11748T）。
