氘同位素效应对交替 GC 寡核苷酸中激发态动力学的影响。
Deuterium isotope effect on excited-state dynamics in an alternating GC oligonucleotide.
机构信息
Department of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA.
出版信息
J Am Chem Soc. 2009 Dec 9;131(48):17557-9. doi: 10.1021/ja9076364.
Abstract
Isotope effects on the excited-state dynamics of single- and double-stranded GC-containing DNAs were studied by femtosecond transient absorption spectroscopy. A pronounced deuterium isotope effect was observed in alternating d(GC)(9).d(GC)(9), but none was seen in the nonalternating or single-stranded variations investigated. These findings demonstrate that an interstrand process involving proton-coupled electron transfer contributes to the excited-state dynamics in DNAs having an appropriate base sequence.
摘要
通过飞秒瞬态吸收光谱研究了含单链和双链 GC 的 DNA 在激发态动力学上的同位素效应。在交替的 d(GC)(9).d(GC)(9) 中观察到明显的氘同位素效应，但在研究的非交替或单链变化中则没有观察到。这些发现表明，涉及质子耦合电子转移的链间过程有助于具有适当碱基序列的 DNA 的激发态动力学。
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