Hospital for Sick Children, University of Toronto, ON, Canada.
Cell Stem Cell. 2009 Dec 4;5(6):610-23. doi: 10.1016/j.stem.2009.10.019.
Despite the remarkable regenerative capacity of mammalian skin, an adult dermal stem cell has not yet been identified. Here, we investigated whether skin-derived precursors (SKPs) might fulfill such a role. We show that SKPs derive from Sox2(+) hair follicle dermal cells and that these two cell populations are similar with regard to their transcriptome and functional properties. Both clonal SKPs and endogenous Sox2(+) cells induce hair morphogenesis, differentiate into dermal cell types, and home to a hair follicle niche upon transplantation. Moreover, hair follicle-derived SKPs self-renew, maintain their multipotency, and serially reconstitute hair follicles. Finally, grafting experiments show that follicle-associated dermal cells move out of their niche to contribute cells for dermal maintenance and wound-healing. Thus, SKPs derive from Sox2(+) follicle-associated dermal precursors and display functional properties predicted of a dermal stem cell, contributing to dermal maintenance, wound-healing, and hair follicle morphogenesis.
尽管哺乳动物的皮肤具有显著的再生能力，但尚未鉴定出成年皮肤干细胞。在这里，我们研究了皮肤衍生前体细胞（SKP）是否可能发挥这种作用。我们表明，SKP 来源于 Sox2(+)毛囊真皮细胞，这两种细胞群在其转录组和功能特性方面相似。克隆 SKP 和内源性 Sox2(+)细胞都诱导毛发生成，分化为真皮细胞类型，并在移植后归巢到毛囊龛位。此外，毛囊衍生的 SKP 自我更新，保持其多能性，并连续重建毛囊。最后，移植实验表明，毛囊相关真皮细胞离开其龛位，为真皮维持和伤口愈合提供细胞。因此，SKP 来源于 Sox2(+)毛囊相关真皮前体，并表现出预测的真皮干细胞功能特性，有助于真皮维持、伤口愈合和毛囊形态发生。
