Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03, Faculté de Pharmacie de Monastir, Monastir, Tunisia.
Food Chem Toxicol. 2010 Feb;48(2):710-5. doi: 10.1016/j.fct.2009.11.053. Epub 2009 Nov 29.
The present study was undertaken to provide a set of data on the safety of an aqueous extract (AQE) from Moricandia arvensis. For this reason, Escherichia coli tested strains PQ35 and PQ37 were used to detect induction of DNA lesions by AQE. The SOS Chromotest showed that AQE induced a marginally genotoxic effect, as expressed by the induction factor (IF) value only with E. coli PQ37 tested strain (IF=1.77 at a dose of 250 microg/assay). The measurement of the anti-genotoxic activity of the AQE was also studied by inhibition of beta-galactosidase induction. A significant anti-genotoxic effect was observed with different tested doses of AQE, which suggests that M. arvensis extract has the potential to protect DNA from the action of nitrofurantoïn (NF) and free radicals generated by hydrogen peroxide (H2O2). In addition to anti-genotoxic activity, AQE showed a free-radical-scavenging capacity towards ABTS+* and DPPH*. Total phenolic content was also evaluated following Folin-Ciocalteu method and results indicated high correlation between total phenol content and anti-genotoxic and antioxidant activities for AQE, but the highest correlation was showed with its capacity to stabilize ABTS+* (R2=0.9944).
本研究旨在提供一组有关 Moricandia arvensis 水提物（AQE）安全性的数据。为此，使用大肠杆菌测试菌株 PQ35 和 PQ37 来检测 AQE 是否诱导 DNA 损伤。SOS 显色试验表明，AQE 仅在测试菌株 PQ37 中诱导出轻微的遗传毒性效应，表现为诱导因子（IF）值为 1.77（在 250μg/试验剂量下）。还通过抑制β-半乳糖苷酶诱导来研究 AQE 的抗遗传毒性活性。用不同测试剂量的 AQE 观察到显著的抗遗传毒性作用，这表明 M. arvensis 提取物具有保护 DNA 免受呋喃妥因（NF）和过氧化氢（H2O2）产生的自由基作用的潜力。除了抗遗传毒性活性外，AQE 对 ABTS+和 DPPH也具有清除自由基的能力。还采用 Folin-Ciocalteu 法评估了总酚含量，结果表明 AQE 的总酚含量与其抗遗传毒性和抗氧化活性之间存在高度相关性，但与稳定 ABTS+*的能力相关性最高（R2=0.9944）。
