Biomedical Institute, Research Center, Hayashibara Biochemical Laboratories, Inc., 675-1 Fujisaki, Naka-ku, Okayama 702-8006, Japan.
Biol Pharm Bull. 2009 Dec;32(12):2022-8. doi: 10.1248/bpb.32.2022.
We examined the hypolipidemic effect of propolis in a mouse obesity model induced by a high fat-diet. C57BL/6N mice were fed a high-fat diet ad libitum and given propolis extract intragastrically at 0 mg/kg (control), 5 mg/kg or 50 mg/kg twice daily for 10 d. Compared with mice in the control group, mice in the propolis extract-administrated groups displayed a reduction in all of the following parameters: body weight gain, weight of visceral adipose tissue, liver and serum triglycerides, cholesterol, and non-esterified fatty acids. Real-time polymerase chain reaction analysis of the liver showed down-regulation of mRNA expression associated with fatty acid biosynthesis, including fatty acid synthase, acetyl-CoA carboxylase alpha, and sterol regulatory element binding protein in the propolis-administrated mice. Subsequently, obese C57BL/6N mice that had been administered a high-fat diet were given propolis extract at 0 mg/kg (control), 2.5 mg/kg or 25 mg/kg for 4 weeks. The propolis extract treated mice showed a decrease in weight gain, a reduction of serum non-esterified fatty acids, and lipid accumulation in the liver. These results suggest that propolis extract prevented and mitigated high-fat diet-induced hyperlipidemia by down-regulating the expression of genes associated with lipid metabolism.
我们在高脂肪饮食诱导的肥胖小鼠模型中研究了蜂胶的降血脂作用。C57BL/6N 小鼠自由摄入高脂肪饮食，并连续 10 天每天两次给予蜂胶提取物 0mg/kg（对照组）、5mg/kg 或 50mg/kg。与对照组小鼠相比，蜂胶提取物给药组小鼠的以下参数均降低：体重增加、内脏脂肪组织、肝脏和血清甘油三酯、胆固醇和非酯化脂肪酸的重量。肝脏的实时聚合酶链反应分析显示，与脂肪酸生物合成相关的 mRNA 表达下调，包括脂肪酸合酶、乙酰辅酶 A 羧化酶 α 和固醇调节元件结合蛋白在蜂胶给药小鼠中。随后，给予高脂肪饮食的肥胖 C57BL/6N 小鼠连续 4 周给予 0mg/kg（对照组）、2.5mg/kg 或 25mg/kg 的蜂胶提取物。蜂胶提取物处理的小鼠体重增加减少，血清非酯化脂肪酸减少，肝脏脂质堆积减少。这些结果表明，蜂胶提取物通过下调与脂质代谢相关的基因表达来预防和减轻高脂肪饮食诱导的高脂血症。
